| HPLC/MSn法鉴定环境激素邻苯二甲酸丁基苄酯在小鼠尿中的代谢产物 |
| |
| 引用本文： | 李文兰,季宇彬,张大雷,范玉奇.HPLC/MSn法鉴定环境激素邻苯二甲酸丁基苄酯在小鼠尿中的代谢产物[J].环境科学,2007,28(3):627-632. |
| |
| 作者姓名： | 李文兰 季宇彬 张大雷 范玉奇 |
| |
| 作者单位： | 哈尔滨商业大学生命科学与环境科学研究发展中心,哈尔滨150076;哈尔滨商业大学生命科学与环境科学研究发展中心,哈尔滨150076;黑龙江省公安厅,哈尔滨150008;哈尔滨商业大学生命科学与环境科学研究发展中心,哈尔滨150076 |
| |
| 基金项目： | 黑龙江省博士后基金项目 |
| |
| 摘 要： | 应用HPLC-MSn方法,研究了邻苯二甲酸丁基苄酯在小鼠尿液中的代谢产物,并对尿中推测的代谢物和标准品分别进行HPLC-UV和二级全扫描质谱（MS2）分析,结果在尿中发现了6个代谢产物,分别为邻苯二甲酸、马脲酸、邻苯二甲酸单丁酯、邻苯二甲酸苄酯、邻苯二甲酸单丁酯葡萄糖醛酸结合物及邻苯二甲酸苄酯葡萄糖醛酸结合物.推断环境激素邻苯二甲酸丁基苄酯在小鼠体内的代谢途径为原形首先水解生成邻苯二甲酸单丁酯、邻苯二甲酸苄酯、邻苯二甲酸,部分邻苯二甲酸发生脱羧反应生成苯甲酸,苯甲酸再与内源性物质甘氨酸结合成马脲酸,邻苯二甲酸单丁酯、邻苯二甲酸苄酯和内源性β-D-葡萄糖醛酸结合,生成水溶性较大的邻苯二甲酸单丁酯葡萄糖醛酸结合物和邻苯二甲酸苄酯葡萄糖醛酸结合物.
|
| 关 键 词： | 环境激素 邻苯二甲酸丁基苄酯 液相色谱/质谱法 代谢产物 |
| 文章编号： | 0250-3301（2007）03-0627-06 |
| 收稿时间： | 2006/4/26 0:00:00 |
| 修稿时间： | 6/5/2006 12:00:00 AM |
Identification of Metabolites of Environmental Hormone Butylbenzyl Phthalate in Mice Urine by Liquid Chromatography/Ion Trap Mass Spectrometry |
| |
| LI Wen-lan,JI Yu-bin,ZHANG Da-lei and FAN Yu-qi.Identification of Metabolites of Environmental Hormone Butylbenzyl Phthalate in Mice Urine by Liquid Chromatography/Ion Trap Mass Spectrometry[J].Chinese Journal of Environmental Science,2007,28(3):627-632. |
| |
| Authors: | LI Wen-lan JI Yu-bin ZHANG Da-lei and FAN Yu-qi |
| |
| Institution: | 1. Center of Research and Development on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China;2.Public Security of Heilongjiang Province, Harbin 150008,China |
| |
| Abstract: | The metabolites of butylbenzyl phthalate in mice urine were identified by HPLC-MS(n). After the analysis of speculated metabolites and standard sample by HPLC-UV and the second full mass scanning (MS2), we find six metabolites: phthalic acid, benzoyl glycocoll, monobutyl phthalate, benzyl phthalate, glucuronyl-monobutyl phthalate, glucuronyl-benzyl phthalate. We deduced metabolic approach of butyl benzyl phthalate in vivo was that butyl benzyl phthalate hydrolyzed into the phthalic acid, monobutyl phthalate and benzyl phthalate. Partial phthalic acid became into benzoic acid by decarboxylizing. Then benzoic acid conjugated endogenous glycin and benzoyl glycocoll was produced. Monobutyl phthalate and benzyl phthalate conjugated endogenous beta-D-glucuronic acid and produced the glucurony-monobutyl phthalate and glucuronyl-benzyl phthalate. |
| |
| Keywords: | environmental hormone butylbenzyl phthalate HPLC/MS metabolites |
| 本文献已被 CNKI 维普 PubMed 等数据库收录！ |
| 点击此处可从《环境科学》浏览原始摘要信息 |
| 点击此处可从《环境科学》下载免费的PDF全文 |
|
