| 溴酸盐对普通小球藻的生长以及生理特性的影响 |
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| 引用本文： | 王执伟,刘冬梅,张文娟,崔福义.溴酸盐对普通小球藻的生长以及生理特性的影响[J].环境科学,2016,37(6):2158-2163. |
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| 作者姓名： | 王执伟 刘冬梅 张文娟 崔福义 |
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| 作者单位： | 哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090;城市水资源开发利用(北方)国家工程研究中心, 哈尔滨 150090,哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090;城市水资源开发利用(北方)国家工程研究中心, 哈尔滨 150090,哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090;城市水资源开发利用(北方)国家工程研究中心, 哈尔滨 150090,哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090;城市水资源开发利用(北方)国家工程研究中心, 哈尔滨 150090 |
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| 基金项目： | 国家自然科学基金项目(50808052);国家水体污染控制与治理科技重大专项(2012ZX07403-001) |
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| 摘 要： | 在静态暴露的条件下,利用流式细胞术等检测技术,考察了不同浓度的溴酸盐对普通小球藻的生长以及生理特性的影响,并对溴酸盐的毒性作用机制进行了初步的探索.结果表明,当溴酸盐浓度达到8 mmol·L~(-1),持续暴露96 h时,能够引起普通小球藻比生长率显著降低、细胞膜完整性显著降低、酯酶活性显著升高、线粒体膜电位显著升高、活性氧水平显著增加.扫描电镜可直观表明细胞膜受到损伤.由此说明,由于溴酸盐毒性的持续作用,使得活性氧在藻细胞内积累,普通小球藻自身的调节功能不能及时消除过多的活性氧,从而引起细胞膜完整性、酯酶活性和线粒体膜电位出现异常情况,藻细胞生理功能出现紊乱,藻细胞结构遭到破坏,最终导致普通小球藻生长受到抑制或死亡.
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| 关 键 词： | 溴酸盐 普通小球藻 流式细胞术 生理特性 致毒机制 |
| 收稿时间： | 2015/12/6 0:00:00 |
| 修稿时间： | 1/4/2016 12:00:00 AM |
Effects of Bromate on the Growth and Physiological Characteristics of Chlorella vulgaris |
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| WANG Zhi-wei,LIU Dong-mei,ZHANG Wen-juan and CUI Fu-yi.Effects of Bromate on the Growth and Physiological Characteristics of Chlorella vulgaris[J].Chinese Journal of Environmental Science,2016,37(6):2158-2163. |
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| Authors: | WANG Zhi-wei LIU Dong-mei ZHANG Wen-juan and CUI Fu-yi |
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| Institution: | State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;National Engineering Research Center of Urban Water Resources, Harbin 150090, China,State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;National Engineering Research Center of Urban Water Resources, Harbin 150090, China,State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;National Engineering Research Center of Urban Water Resources, Harbin 150090, China and State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;National Engineering Research Center of Urban Water Resources, Harbin 150090, China |
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| Abstract: | The effects of bromate on the growth and physiological characteristics of Chlorella vulgaris were investigated via the static exposure experiments and tested by flow cytometry. The results showed that when Chlorella vulgaris was continuously exposed to bromate for 96 h at 8 mmol·L-1, the specific growth rates and cell membrane integrity decreased significantly, while the esterase activity, mitochondrial membrane potential and reactive oxygen species (ROS) increased significantly. The membrane-damaged cells could be found from the scanning electron microscopy analysis. It could be identified that ROS were overproduced in presence of bromate, which could not be eliminated by Chlorella vulgaris in time through self-regulation. The excess ROS could lead to abnormal situation of cell membrane integrity, esterase activity and mitochondrial membrane potential, disorder of physiology function and damage of cell structure. It could be concluded that Chlorella vulgaris died or their growth was inhibited by the existence of bromate. |
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| Keywords: | bromate Chlorella vulgaris flow cytometry physiological characteristics toxicity mechanism |
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