好氧亚硝化颗粒污泥中硝化细菌群落结构分析

在小试好氧上流式污泥床(AUSB)反应器中,实现了由厌氧颗粒污泥到好氧硝化污泥再到亚硝化颗粒污泥的转化,AUSB反应器的亚硝化率稳定在90%以上.利用FISH、荧光实时定量PCR等技术,考察了AUSB反应器中好氧颗粒污泥中硝化菌群的生态分布.结果表明:好氧亚硝化颗粒污泥呈层状结构,氨氧化细菌(AOB)主要分布在颗粒污泥表层,亚硝酸盐氧化细菌(NOB)多分布在内层,颗粒内核则无活性细胞;随反应器氨氮负荷逐渐提高,颗粒污泥中AOB的相对含量逐渐升高,当NH₃-N负荷分别为0、0.4、1、

Community Structure of Nitrification Bacteria in Aerobic Short-Cut Nitrification Granule

By using a lab-scale aerated upflow sludge bed reactor,the inoculated anaerobic granule was cultivated to aerobic nitrification granule,and then converted to short-cut nitrification granule with the short-cut nitrification efficiencies above 90%.Appling real-time quantity PCR,and florescent in situ hybridization techniques,the ecological community structure of nitrification bacteria in aerobic granules were studied.The results show that there existed a layered structure in the aerobic granule,the ammonia oxidizing bacteria(AOB) was mainly located in the surface area of the granule,and the nitrite oxidizing bacteria(NOB) was mainly located in the inner area of the granule,was just adjacent to the AOB.There was no active bacteria in the inner core area.The amount of AOB in the granules increased,as the ammonia loading rate of the reactor was increased gradually.The percentage of AOB in the total amount of Eubacteria in the granule was 0.45%,5.20%,15.37%,48.55% respectively,as the ammonia loading rate of the reactor were 0,0.4,1.0 and 2.2 kg/(m³·d) respectively,and the nitrosofication efficiency were 0%,35%,50%,99% relatively.