EGSB反应器在20℃下处理啤酒废水的工艺及微生物学研究

在20℃下连续运行厌氧膨胀颗粒污泥床(EGSB)反应器,对其处理模拟啤酒废水的工艺运行、污泥特性及微生物种群结构进行研究,结果表明:①HRT为18 h,经184 d的运行,EGSB反应器的有机负荷(以COD计)可达10 kg/(m3·d),COD去除率为85%以上,去除1 kg COD可产沼气0.58 m3;②随有机负荷逐渐提高,颗粒污泥粒径逐渐增大,沉降性能逐渐提高,但当负荷达到10 kg/(m3·d)时,颗粒污泥平均粒径有变小趋势;与中温厌氧污泥相比,低温污泥的胞外多聚物(ECP)总量有较大增加,其中蛋白质含量增加明显;③与接种污泥相比,在20℃下运行不同时期污泥中产甲烷菌的种类未发生明显变化,但接种同样颗粒污泥在15℃下运行6个月后污泥中的优势产甲烷菌种发生了明显的变化.

Performance and microbial characteristics of an EGSB reactor treating brewery wastewater under 20 degrees C

A lab-scale EGSB reactor fed by artificial brewery wastewater was operated under 20 degrees C continuously, and its process performance, characteristics of granular sludge, microbial community structure were investigated. The results indicated that: 1) after 184 days' operation, the organic loading rate of EGSB reactor could reach 10 kg/(m3 x d) at HRT of about 18 h, the COD removal efficiencies were above 85%, and the specific biogas production was about 0.58 m3 per kilogram COD removed. 2) As the organic loading rate increased, the average diameter of the granule increased, and the settling velocity increased too, but when the loading rate reached about 10 kg/(m3 x d), the granular average diameter decreased slightly. Compared with the granular sludge operated at mesophilic condition, the ECP contents especially the protein contents in the granular sludge operated at 20 degrees C increased obviously. 3) Compared with the seed sludge, the main Archaea (methanogenes) species in the sludge samples operated at different loading rates under 20 degrees C did not change greatly, but the dominant methanogenes in the sludge operated under 15 degrees C for 6 months seeded with the same mesophilic sludge changed obviously.