用CBMN法评价4-氯酚与富里酸对CHO细胞的毒性效应

用ＣＨＯ细胞和ＣＢＭ法对４－ＣＰ（４－氯）及春ＦＡ协同作用的细胞毒性效应进行了评价，选用的细胞学毒性参数为总微核率（ＭＮ）、含微核的双核细胞率（ＢＮＭＮ）、核分鲜明指数（ＮＯＩ）和南分裂阻断增殖指数（ＣＢＰＩ），结果表明：ＦＡ单独处理与阴性对照组比较不引起显著性差异；４－Ｃ客ＦＡ联合处理与４－ＣＰ各浓度分别处理相比有显著性的差异；对于ＢＮＭＮ和ＮＤＩ，Ｐ〈０．０１；对于ＭＮ和ＣＢＰＩ，Ｐ〈０．０５

The Assessment of the Cytotoxicity Effects of 4-CP and FA on the CHO Cell by Cytokinesis Block Micronucleus Assay

By means of the cytokinesis block micronucleus(CBMN) assay for the CHO cell,the synergism effect of 4-CP and FA on the cytotoxicity parameters were evaluated.These cytotoxicity parameters include the frequencies of micronuclei (MN),the frequencies of micronucleated binucleated cells (BNMN),the nuclear division index (NDI),and the cytokinesis block proliferation index (CBPI).The results indicated that the cytotoxicity effects with FA(0.66mg·L^-1) alone is not statistically significantly different compared with negative control.The effects of combination of 4-CP at the doses of 5,10,15 and 20mg·L^-1 respectively and FA(0.66mg·L^-1) showed quite significant differences in MN,BNMN (P<0.01),NDI (P<0.01),MN(P<0.05) and CBPI(P<0.05) compared with that of 4-CP at the corresponding four doses respectively.For both treatments (4-CP with FA and 4-CP alone),4-CP at the doses more than 15mg·L^-1 showed a significant differences in MN,BNMN,NDI and CBPI compared with negative control.Also for both treatments the correlativity between the dose of 4-CP and MN,BNMN,NDI and CBPI respectively were statistically significant.This work indicated that it is necessary to control strictly the pollution of chlorophenol in aquatic system due to their synergism effects on cytotoxicity.