简青霉Penicillium simplicissimum木质素降解能力

从土壤中分离得到一株真菌经鉴定为简青霉Penicillium simplicissimum,将该菌用于木质素类化合物利用、染料脱色、天然木素降解及产酶特性等研究,充分证实该菌具有木质素降解能力.简青霉降解木质素是木质素过氧化物酶(LiP)、漆酶(Lac)和木聚糖酶共同作用的结果,降解主要发生在LiP与木聚糖酶活高产的初级代谢阶段,与白腐真菌表现出不同的作用机制.25d培养可使稻草木质素绝对量损失0.23g,降解率达14.94%,同时纤维素、半纤维素也有较高程度的降解.而pH值、Cu²⁺和Mn²⁺浓度对木质素的降解有较大影响.

Lignin Degradation by Penicillium simplicissimum

A strain of fungi was isolated from soil, which was identified as Penicillium simplicissimum. This strain was capable of utilizing several lignin model compounds, making aromatic dyes decoloration and degrading natural lignin. All these results proved that Penicillium simplicissimum has ligninolytic ability. Three kinds of enzymes were believed to be the most important catalyzes in the biodegrading process. They are lignin peroxidase (LiP), laccase (Lac) and hemicellulase. And they always work synergistically. After 25 days' incubation, the amount of rice straw lignin decreased 0.23g, and the degrading rate was 14.94%. Different from the degrading mechanism of the white-rot fungi, the lignin degradation by P.simplicissimum mainly happened during the primary metabolism and it was greatly influenced by the pH of media, the concentration of Cu²⁺ and Mn²⁺.