高活性高耐受甲醛降解菌株的分离鉴定及降解条件研究

以甲醛为唯一碳源,从土壤中分离得到1株甲醛降解菌,经形态学观察、生理生化特性研究和16SrDNA鉴定,该菌株属于恶臭假单胞菌(Pseudomonas putida).通过单因素试验和正交试验考察培养基及培养条件对菌株降解甲醛的影响,得出该菌株降解甲醛的最适条件为:蛋白胨1.2g/L,KH2PO4 4g/L,K2HPO4 3g/L,MgSO4·7H2O 0.2g/L,微量元素母液0.1mL/L,温度30℃,pH值8.在最适降解条件下,分别对不同初始浓度甲醛进行降解试验,结果表明该菌株对甲醛的耐受浓度可达6g/L,54h可将其降解86%,46h可将5g/L甲醛全部降解,35h可全部降解4g/L甲醛.

Isolation, Identification and Conditions of Bacterial Strain Capable to Metabolize High Concentrations of Formaldehyde

One bacterial strain capable to degrade and metabolize formaldehyde as a sole carbon source was isolated from soil. Based on the results of standard morphological identification, physiological and biochemical characters, and 16S rDNA sequence analysis, the strain was identified as Pseudomonas putida. After single factor test and orthogonal test, the optimal condition for formaldehyde degradation was determined as the follows: peptone 1.2 g/L, KH2PO4 4 g/L, K2HPO4 3 g/L, MgSO4 x 7H2O 0.2 g/L, trace elements solution 0.1 mL/L, temperature 30 degrees C, pH 8. Under the optimal conditions, the strain tolerance of original formaldehyde concentration was up to 6 g/L and 86% of formaldehyde was consumed after 54 h. It completely consumed 5 g/L formaldehyde after 46 h and degraded 100% of 4 g/L formaldehyde after 35 h.