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长期施用猪粪水稻土抗生素抗性基因污染研究
引用本文:黄福义,李虎,韦蓓,欧阳纬莹,苏建强.长期施用猪粪水稻土抗生素抗性基因污染研究[J].环境科学,2014,35(10):3869-3873.
作者姓名:黄福义  李虎  韦蓓  欧阳纬莹  苏建强
作者单位:1. 中国科学院城市环境研究所,厦门 361021; 中国科学院大学,北京 100049
2. 中国科学院城市环境研究所,厦门,361021
基金项目:福建省自然科学基金项目,国家自然科学基金项目,科技部国际科技合作项目
摘    要:为了全面研究猪粪有机肥对水稻土抗生素抗性及分布格局的影响,选择295对抗生素抗性基因引物,采用高通量荧光定量PCR对水稻土中的抗生素抗性基因污染情况进行了研究.结果表明,未施用猪粪水稻土检测出66种抗性基因,而施用猪粪水稻土则检测出107种抗生素抗性基因,施用猪粪后水稻土中抗生素抗性基因种类显著增加(P<0.05).相对于未施用猪粪水稻土,施用猪粪水稻土有49种抗生素抗性基因丰度显著增加(P<0.05),其中施用猪粪水稻土中喹诺酮类/氯霉素类抗性基因的mexF的丰度相对于未施用猪粪水稻土增加1791倍.高通量定量结果表明,施用猪粪的水稻土抗生素抗性基因分布格局发生显著变化,高通量定量技术是研究环境抗生素抗性基因的一个非常实用的工具.

关 键 词:猪粪  水稻土  抗生素  抗生素抗性基因  高通量定量PCR
收稿时间:2014/3/13 0:00:00
修稿时间:2014/4/17 0:00:00

Long-Term Manure Application Induced Shift of Diversity and Abundance of Antibiotic Resistance Genes in Paddy Soil
HUANG Fu-yi,LI Hu,WEI Bei,OUYANG Wei-ying and SU Jian-qiang.Long-Term Manure Application Induced Shift of Diversity and Abundance of Antibiotic Resistance Genes in Paddy Soil[J].Chinese Journal of Environmental Science,2014,35(10):3869-3873.
Authors:HUANG Fu-yi  LI Hu  WEI Bei  OUYANG Wei-ying and SU Jian-qiang
Institution:Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;University of Chinese Academy of Sciences, Beijing 100049, China;Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;University of Chinese Academy of Sciences, Beijing 100049, China;Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;University of Chinese Academy of Sciences, Beijing 100049, China;Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China;University of Chinese Academy of Sciences, Beijing 100049, China;Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China
Abstract:To investigate the effect of long-term manure application on the profiles of antibiotic resistance genes (ARGs) in paddy soil, the abundance of ARGs targeted by 295 primers sets were measured by using high-throughput quantitative PCR technique. A total of 107 types of ARGs were detected in long-term manured paddy soil, which is significantly higher than that in control paddy soil (P<0.05). PCA analysis and UPGMA cluster demonstrated that long-term manure application significantly changed the ARGs diversity and abundance in paddy soils. There were 49 types antibiotic resistance genes were enriched significantly in paddy soil applied with manure (P<0.05), especially the mexF, which belongs to quinolones-chloramphenicol resistance genes, increased 1791-fold compared with paddy soil of no manure application. Our results showed that long-term application of manure in paddy soil resulted in the shift of abundance and diversity of ARGs, high-throughput quantitative PCR could be a powerful tool concerning the environmental ARGs dimensions.
Keywords:manure  paddy soil  antibiotic  antibiotic resistance genes  high-throughput qPCR
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