PCR-DGGE技术在城市污水化学生物絮凝处理中的特点

通过PCR-DGGE等分子生物学技术可以不经过常规培养直接从活性污泥和生物膜样品中提取DNA,对16Sr DNA V3区进行PCR扩增,结合DGGE(变性梯度凝胶电泳),从而分析活性污泥与生物膜中微生物种群结构.研究证实,活性污泥培养前后微生物种群结构发生很大的改变.同时对2种污水处理工艺中微生物种群结构进行了对比研究,对同一反应器不同位置微生物分布以及不同工况下的微生物种群结构进行了初步探讨.测定了活性污泥中部分菌种的16S rDNA V3区片段序列,通过NCBI(美国国立生物技术信息中心)基因库比对,初步确定细菌的属.结果显示,PCR-DGGE结合测序技术是一种完全可行的快速进行环境学样品微生物研究的分析方法.

Characteristics of Municipal Sewage Chem-Bioflocculation Treatment Process by Using PCR-DGGE Technology

It is reported that without cultivation, DNA could be directly extracted from environmental samples with molecular biological methods, such as polymerase chain reaction(PCR) and denaturting gradient gel electrophoresis(DGGE). To analyze the community diversity of activated sludge and bio-film in the municipal sewage, work was done to directly extrude crude DNA from activated sludge and bio-film samples, separate and amplify 16S rDNA by PCR and sequence it with DGGE. The results show the significant microbe community difference between cultivated and uncultivated activated sludge. Further research on the community diversity of two different sewage treatment processes was done and initial discussion on the microbial distribution in the same reactor and microbial structure in different experimental conditions was carried out. The sequences of several 16S rDNA DGGE fragments were determined and some possible bacteria were confirmed in comparision in GeneBank(NCBI). The results show that the PCR-DGGE technology combined with sequences determination is a feasible and efficient method for microorganism analysis in environmental sample.