| 微囊藻毒素-LR多克隆抗体的制备 |
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| 引用本文： | 盛建武,何苗,施汉昌,钱易.微囊藻毒素-LR多克隆抗体的制备[J].环境科学,2006,27(4):783-786. |
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| 作者姓名： | 盛建武 何苗 施汉昌 钱易 |
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| 作者单位： | 清华大学环境科学与工程系环境模拟与污染控制国家重点联合实验室,北京,100084 |
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| 基金项目： | 国家高技术研究发展计划(863)项目(2002AA649160) |
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| 摘 要： | 通过对新西兰大白兔免疫自制的微囊藻毒素-LR(Microcystin-LR,MC-LR)完全抗原BSA-MC-LR,获得了质量较好的抗MC-LR的多克隆抗体,间接ELISA表明抗体的效价能达到1.5×105;固定包被抗原OVA-MC-LR,采用间接竞争ELISA测定水体中的微囊藻毒素,标准曲线表明对水样中MC-LR的检测下限为10ng/L,线性区间为30ng/L·3μg/L,能满足对饮用水和地表水中MC-LR的检测要求.
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| 关 键 词： | 微囊藻毒素-LR 多克隆抗体 间接竞争ELISA |
| 文章编号： | 0250-3301（2006）04-0783-04 |
| 收稿时间： | 2005-03-25 |
| 修稿时间： | 2005-03-252005-06-20 |
Preparation of Polyclonal Antibody Against Microcystin-LR |
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| SHENG Jian-wu,HE Miao,HI Han-chang and QIAN Yi.Preparation of Polyclonal Antibody Against Microcystin-LR[J].Chinese Journal of Environmental Science,2006,27(4):783-786. |
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| Authors: | SHENG Jian-wu HE Miao HI Han-chang and QIAN Yi |
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| Institution: | ESPC State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China. |
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| Abstract: | Good quality polyclonal antibody against Microcystin-LR (MC-LR) was obtained from the New Zealand rabbit immunized with the homemade complete antigen BSA-MC-LR. Indirect ELISA shows the titer of this antibody is more than 1.5 x 10(5); Indirect competitive ELISA immobilized the coating antigen OVA-MC-LR was adapted to detect MCs, the calibration curve shows that the detection lower limit is 10 ng/L, and the range of quantitative detection was between 30 ng/L to 3 microg/L, with a good linearity, which will well potentially suit for sensitive analysis for MC-LR in drinking as well as surface water samples. |
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| Keywords: | Microcystin-LR polyclonal antibody indirect competitive ELISA |
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