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石油污染地下水中细菌多样性研究
引用本文:郝春博,王广才,董健楠,张倩,蔡五田.石油污染地下水中细菌多样性研究[J].环境科学,2009,30(8):2464-2472.
作者姓名:郝春博  王广才  董健楠  张倩  蔡五田
作者单位:1. 中国地质大学(北京)水资源与环境学院,北京,100083;中国地质大学(北京)地质微生物学实验室,北京,100083
2. 中国地质大学(北京)水资源与环境学院,北京,100083
3. 中国地质调查局水文地质环境地质调查中心,保定,071051
基金项目:国家自然科学基金项目(40802059);;中国地质调查局地质调查工作项目(1212010634606)
摘    要:采集了某废弃炼油厂的石油污染地下水样品,提取水中微生物总DNA,构建细菌16S rDNA克隆文库,并通过16S rDNA序列的系统发育分析,对样品中的细菌种群多样性以及群落结构进行了研究.结果表明,文库中阳性克隆的16S rDNA序列分属10个细菌类群,分别为γ-Proteobacteria(49.1%)、α-Proteobacteria(12.9%)、β-Proteobacteria(11.1%)、Bacteroidetes(9.2%)、Verrucomicrobia(6.7%)、Acidobacteria(2.5%)、δ-Proteobacteria(1.2%)、Actinobacteria(1.2%)、Planctomycetes(0.6%)、Unidentifiedbacteria(5.5%).在这一生态系统中,γ-Proteobacteria类细菌占据主导地位,接近50%,尤其是假单胞菌属(Pseudomonas)微生物在文库中的比例达35.6%.该石油污染地下水样品中细菌与许多其它已知的降解菌亲缘关系较近,如鞘胺醇单胞菌(Sphingomonas)、红球菌(Rhodococcus)和短波单胞菌(B...

关 键 词:石油污染  地下水  16S  rDNA  克隆文库  细菌多样性  
收稿时间:2008/10/21 0:00:00
修稿时间:2008/12/14 0:00:00

Bacterial Biodiversity in the Groundwater Contaminated by Oil
HAO Chun-bo,WANG Guang-cai,DONG Jian-nan,ZHANG Qian and CAI Wu-tian.Bacterial Biodiversity in the Groundwater Contaminated by Oil[J].Chinese Journal of Environmental Science,2009,30(8):2464-2472.
Authors:HAO Chun-bo  WANG Guang-cai  DONG Jian-nan  ZHANG Qian and CAI Wu-tian
Institution:1;2;1.School of Water Resources and Environment;China University of Geosciences;Beijing 100083;China;2.Laboratory of Geomicrobiology;3.Centre for Hydrogeology and Environmental Geology;China Geological Survey;Baoding 071051;China
Abstract:An oil-contaminated groundwater sample was collected at an abandoned petrochemical factory. Bacterial genome DNA was extracted for the 16S rDNA gene amplification, and then a bacterial 16S rDNA gene clone library was constructed. After the phylogenetic analysis of 16S rDNA sequences, bacterial diversity and community structure of the groundwater were studied. The results showed the bacteria in the groundwater could be divided into 10 groups, which were as follows: γ-Proteobacteria (49. 1% ), α-Proteobacteria ( 12.9% ), β-Proteobacteria (11.1% ) , Bacteroidetes (9.2% ), Verrucomicrobia (6.7% ), Acidobacteria ( 2.5% ) , δ-Proteobacteria ( 1.2% ) , Actinobacteria ( 1.2% ), Planctomycetes (0.6% ), Unidentified bacteria(5.5% ) . γ- Proteobacteria was predominant in the ecosystem (near 50% of total clones),and especially Pseudomonas accounted for 35.6 % of all bacteria. Many clones also affiliated with other degrading bacteria, such as Sphingomonas , Rhodococcus, Brevundimonas . Furthermore, a lot of 16S rDNA sequences in the clone library had high similarity to the ones originated from similar polluted environments, such as soil and groundwater contaminated with chlorinated hydrocarbon, soil and groundwater contaminated with polycyclic aromatic hydrocarbon, soil contaminated with PCBs, antibiotics production wastewater and activated sludge, which proved that there were lots of degrading bacteria population in the oil-contaminated water.
Keywords:oil-contaminated  groundwater  16S rDNA  clone library  bacterial diversity  
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