| 农业废物好氧堆肥中氨氧化细菌的群落结构 |
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| 引用本文： | 虞泳,曾光明,陈耀宁,张嘉超,余震,刘智峰,赵明杰,胡春晓.农业废物好氧堆肥中氨氧化细菌的群落结构[J].环境科学,2011,32(10):3067-3072. |
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| 作者姓名： | 虞泳 曾光明 陈耀宁 张嘉超 余震 刘智峰 赵明杰 胡春晓 |
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| 作者单位： | 湖南大学环境科学与工程学院,长沙410082／环境生物与控制教育部重点实验室（湖南大学）,长沙410082 |
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| 基金项目： | 国家教育部长江学者和创新团队发展计划项目(IRT0719)；湖南重大专项(2009FJ1010)；湖南省研究生创新项目；湖南省自然科学创新研究群体基金项目（10JJ7005）；国家自然科学基金项目（50978088） |
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| 摘 要： | 应用聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）技术对农业废物好氧堆肥过程中氨氧化细菌（ammonia-oxidizingbacteria,AOB）种群结构随时间的变化情况进行了研究,结果表明,AOB群落的Shannon-Weaver指数初始值为2.58,堆肥结束时为2.02,多样性整体呈下降趋势.通过对部分优...
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| 关 键 词： | 堆肥 氨氧化细菌 16S rDNA PCR-DGGE 冗余分析 |
| 收稿时间： | 2010/12/21 0:00:00 |
| 修稿时间： | 4/5/2011 12:00:00 AM |
Succession of Ammonia-Oxidizing Bacteria Community Structure During Agricultural Waste Composting |
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| YU Yong,ZENG Guang-ming,CHEN Yao-ning,ZHANG Jia-chao,YU Zhen,LIU Zhi-feng,ZHAO Ming-jie and HU Chun-xiao.Succession of Ammonia-Oxidizing Bacteria Community Structure During Agricultural Waste Composting[J].Chinese Journal of Environmental Science,2011,32(10):3067-3072. |
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| Authors: | YU Yong ZENG Guang-ming CHEN Yao-ning ZHANG Jia-chao YU Zhen LIU Zhi-feng ZHAO Ming-jie and HU Chun-xiao |
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| Institution: | YU Yong1,2,ZENG Guang-ming1,CHEN Yao-ning1,ZHANG Jia-chao1,YU Zhen1,LIU Zhi-feng1,ZHAO Ming-jie1,HU Chun-xiao1,2(1.College of Environmental Science and Engineering,Hunan University,Changsha 410082,China,2.Key Laboratory of Environmental Biology and Pollution Control(Hunan University),Ministry of Education,China) |
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| Abstract: | The dynamic succession of ammonia-oxidizing bacteria(AOB)community during the agricultural waste composting was investigated using the polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE).The changes of pile temperature,pH,moisture content,soluble NH+4-N and NO-3-N were measured.DGGE results showed that the Shannon-Weaver index changed from 2.58 to 2.02 during the composting process,which reflected the diversity trends downward overall.Nitrosospira and Nitrosomonas were dominant speci... |
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| Keywords: | composting ammonia-oxidizing bacteria(AOB) 16S rDNA PCR-DGGE redundancy analysis(RDA) |
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