| 堆肥过程多阶段强化接种对细菌群落多样性的影响 |
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| 引用本文： | 党秋玲,李鸣晓,席北斗,魏自民,刘驰,夏训峰,杨天学,李晔.堆肥过程多阶段强化接种对细菌群落多样性的影响[J].环境科学,2011,32(9):2689-2695. |
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| 作者姓名： | 党秋玲 李鸣晓 席北斗 魏自民 刘驰 夏训峰 杨天学 李晔 |
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| 作者单位： | 中国环境科学研究院水环境系统工程研究室，北京 100012； 东北农业大学生命科学学院，哈尔滨 150030;东北农业大学生命科学学院，哈尔滨 150030;中国环境科学研究院水环境系统工程研究室，北京 100012;东北农业大学生命科学学院，哈尔滨 150030;中国环境科学研究院水环境系统工程研究室，北京 100012;中国环境科学研究院水环境系统工程研究室，北京 100012;中国环境科学研究院水环境系统工程研究室，北京 100012;东北农业大学生命科学学院，哈尔滨 150030 |
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| 基金项目： | 国家自然科学基金项目（50878201）；国家科技支撑计划课题项目（2006BAC06B04，2008BADC4B00，2009BADC2B04）；农业科技成果转化资金项目（2008GB24420470） |
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| 摘 要： | 以生活垃圾为原料进行好氧堆肥,采用多阶段的方式强化接种功能微生物菌剂,利用PCR-DGGE方法并结合聚类分析和Shannon-Weaver指数变化来研究堆肥过程中多阶段强化接种对细菌群落多样性的影响,同时监测木质纤维素降解率的变化.结果表明,多阶段接种堆肥能有效提高堆体降温期和二次发酵期的温度;和普通接种堆肥相比可使半...
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| 关 键 词： | 堆肥 多阶段接种 接种菌剂 PCR-DGGE 细菌群落多样性 |
| 收稿时间： | 2010/10/7 0:00:00 |
| 修稿时间： | 2010/12/8 0:00:00 |
Effects of Multi-stage Strengthening Inoculation on Bacterial Community Diversity During Composting |
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| DANG Qiu-ling,LI Ming-xiao,XI Bei-dou,WEI Zi-min,LIU Chi,XIA Xun-feng,YANG Tian-xue and LI Ye.Effects of Multi-stage Strengthening Inoculation on Bacterial Community Diversity During Composting[J].Chinese Journal of Environmental Science,2011,32(9):2689-2695. |
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| Authors: | DANG Qiu-ling LI Ming-xiao XI Bei-dou WEI Zi-min LIU Chi XIA Xun-feng YANG Tian-xue and LI Ye |
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| Institution: | DANG Qiu-ling1,2,LI Ming-xiao2,XI Bei-dou1,WEI Zi-min2,LIU Chi1,XIA Xun-feng1,YANG Tian-xue1,LI Ye2(1.Laboratory of Water Environmental System Engineering,Chinese Research Academy of Environmental Science,Beijing 100012,China,2.College of Life Science,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | Effects of multi-stage strengthening inoculation on bacterial community diversity were analyzed using PCR-DGGE method in municipal solid waste composting combined with Cluster analysis and changes of Shannon-Weaver index, and the changes of lignocellulose's degradation rate were also detected during the process. The results showed that Multi-stage inoculation of composting can improve the temperature of cooling and the secondary fermentation period. And the hemicellulose, cellulose and lignin degradation rate increased by 7.19%,10.89% and 8.98% compared to general inoculation composting. The analysis of bacterial community diversity indicated that the diversity index of the two inoculation showed significant differences, the microbial inoculation could live well in the pile. It could avoid competition between different inoculated agents and competition between inoculated and indigenous microorganisms, and could improve bacterial community diversity during composting especially for maturity stage.It could speed up the composting process of stabilization. |
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| Keywords: | composting multi-stage inoculation microbial inoculation agents PCR-DGGE diversity of bacteria community |
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