| 酸性矿山废水区域沉积物中嗜酸菌多样性研究 |
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| 引用本文： | 郝春博,张洪勋,白志辉,张保国,张徐祥.酸性矿山废水区域沉积物中嗜酸菌多样性研究[J].环境科学,2006,27(11):2255-2260. |
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| 作者姓名： | 郝春博 张洪勋 白志辉 张保国 张徐祥 |
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| 作者单位： | 1. 中国科学院生态环境研究中心环境生物技术室,北京,100085
2. 南京大学环境学院污染控制与资源化研究国家重点实验室,南京,210093 |
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| 基金项目： | 中国科学院知识创新工程项目(KJCX2-SW-206-4) |
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| 摘 要： | 采集了酸性矿山废水区域酸性沉积物样品,运用16S rDNA克隆文库技术和变性梯度凝胶电泳(DGGE)技术,研究了沉积物中的嗜酸菌类群以及它们的群落结构.结果表明,沉积物中的嗜酸菌类型较新,大多数16S rDNA序列与已知序列的相似性均在97%以下.这些嗜酸菌主要为Acidobacteria,β/-γProteobacteria,δ-Proteobacteria,Nitrospira,Candidate Division TM7,low G+C Gram-positive类微生物.在这一酸性生态系统中,δ-Proteobacteria类细菌占据主导地位.对酸性沉积物中嗜酸菌多样性的研究有利于发现高效处理酸性矿山废水的方法.
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| 关 键 词： | 酸性矿山废水 沉积物 嗜酸菌 16SrDNA 变性梯度凝胶电泳 |
| 文章编号： | 0250-3301（2006）11-2255-06 |
| 收稿时间： | 2005-11-25 |
| 修稿时间： | 1/9/2006 12:00:00 AM |
Biodiversity of Acidophiles in the Sediment at an Acid Mine Drainage Site |
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| Hao Chun-Bo,Zhang Hong-Xun,Bai Zhi-Hui,Zhang Bao-Guo,Zhang Xu-Xiang.Biodiversity of Acidophiles in the Sediment at an Acid Mine Drainage Site[J].Chinese Journal of Environmental Science,2006,27(11):2255-2260. |
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| Authors: | Hao Chun-Bo Zhang Hong-Xun Bai Zhi-Hui Zhang Bao-Guo Zhang Xu-Xiang |
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| Institution: | 1. Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; 2. State Key Laboratory of Pollution Control and Resource Reuse, Nanjing University, Nanjing 210093, China |
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| Abstract: | A sediment sample was collected at an acid mine drainage site in Anhui. The acidophiles composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis (DGGE). The results indicated that the acidophiles in the sediment were novel compared with the data in GenBank. The acidophiles affiliated with Acidobacteria, beta/gamma-Proteobacteria, delta-Proteobacteria, Nitrospira, Candidate Division TM7, low G + C Gram-positive. delta-Proteobacteria were major group in this acidic ecosystem. The research of aicdophiles community in sediment can help us develop high efficient method to treat acid mine drainage (AMD). |
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| Keywords: | acid mine drainage sediment acidophile 16S rDNA DGGE |
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