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胞外多聚物在好氧颗粒污泥形成中的作用机制
引用本文:张丽丽,陈效,陈建孟,蔡伟民.胞外多聚物在好氧颗粒污泥形成中的作用机制[J].环境科学,2007,28(4):795-799.
作者姓名:张丽丽  陈效  陈建孟  蔡伟民
作者单位:1. 浙江工业大学生物与环境工程学院,杭州,310032
2. 上海交通大学环境科学与工程学院,上海,200240
摘    要:在SBR反应器活性污泥好氧颗粒化过程中,分析不同时期污泥EPS主要成分的变化、污泥表面特性的变化及二者相关关系.不同时期污泥样品胞外蛋白的SDS-PAGE表明,蛋白分子量条带主要分布在(31.0~97.4)×103,与种泥相比,颗粒污泥在形成过程中增加了一些新蛋白条带,且条带颜色逐渐变深,定性表明蛋白种类及含量的增加.定量测定也表明,胞外蛋白分泌量随颗粒污泥的形成逐渐从49.4 mg·g-1增至148.3 mg·g-1,多糖则无明显变化,蛋白/多糖值也从2.3逐渐增至4.9.颗粒污泥表面疏水性比种泥约增加1倍,疏水性的变化与蛋白/多糖值正相关,相关系数为0.969.测得种泥与颗粒污泥表面Zeta电位平均值分别为-28.5 mV和-13.2 mV,颗粒污泥表面的电负性明显降低.由蛋白质自身特性,可推测其通过增加污泥表面相对疏水性和降低污泥表面电负性而促进好氧颗粒污泥形成的作用机制.

关 键 词:胞外多聚物  胞外蛋白  好氧颗粒污泥  表面特性  机制
文章编号:0250-3301(2007)04-0795-05
收稿时间:4/8/2006 12:00:00 AM
修稿时间:2006-04-082006-06-19

Role Mechanism of Extracellular Polymeric Substances in the Formation of Aerobic Granular Sludge
ZHANG Li-li,CHEN Xiao,CHEN Jian-meng and CAI Wei-min.Role Mechanism of Extracellular Polymeric Substances in the Formation of Aerobic Granular Sludge[J].Chinese Journal of Environmental Science,2007,28(4):795-799.
Authors:ZHANG Li-li  CHEN Xiao  CHEN Jian-meng and CAI Wei-min
Institution:1. School of Biological and Environmental Engieering, Zhejiang University of Technology, Hangzhou 310032, China; 2. School of Environmental Science and Engineering, Shanghai Jiaotong University, Shanghai 200240, China
Abstract:During the aerobic granulation of activated sludge in SBR, the changes in the key components of sludge EPS (extracellular polymeric substances) and surface characteristics, and the correlation between them were analyzed. The SDS-PAGE results of exopolymeric protein for different sludge samples indicated that the distribution of bands for protein molecular weight was in the range of 31.0 x 10(3) - 97.4 x 10(3). Compared with seed sludge, some new protein bands increased with the aerobic granulation, and the color of the bands were enhanced, indicating the species and contents of exopolymeric protein gradually increased with the granulation. Moreover the quantitative determination indicated that the protein excreted increased from 49.4 mg x g(-1) to 148.3 mg x g(-1) with the granulation. There was no obvious increase in the polysaccharide content. The PN/PS ratio was increased from to 2.3 to 4.9 accordingly. The cell hydrophobicity of aerobic granular sludge was 1 time higher than that of seed sludge. The changes in cell hydrophobicity were positively correlated with PN/PS values, and the related coefficient was 0.969. The average Zeta potential of seed sludge and granular sludge was -28.5 mV and -13.2 mV, respectively. Obviously, the surface negative charges of granular sludge decreased. From the protein characteristics, we speculate the increase in exopolymeric protein content may enhance cell relative hydrophobicity and reduce negative surface charges, thus contributing to aerobic granulation.
Keywords:extracellular polymeric substances(EPS)  exopolymeric protein  aerobic granular sludge  surface characteristics  mechanism
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