| 降解蒽嗜盐菌AD-3的筛选、降解特性及加氧酶基因的研究 |
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| 引用本文： | 崔长征,冯天才,于亚琦,董婓,杨昕梅,冯耀宇,刘勇弟,林汉平.降解蒽嗜盐菌AD-3的筛选、降解特性及加氧酶基因的研究[J].环境科学,2012,33(11):4062-4068. |
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| 作者姓名： | 崔长征 冯天才 于亚琦 董婓 杨昕梅 冯耀宇 刘勇弟 林汉平 |
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| 作者单位： | 1. 华东理工大学资源与环境工程学院,国家环境保护化工过程环境风险评价与控制重点实验室,上海200237
2. 国家知识产权局专利局专利审查协作北京中心,北京,100081 |
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| 基金项目： | 环境模拟与污染控制国家重点联合实验室开放基金项目(11K02ESPCT);中央高校基本科研业务费专项资金项目(WB1114031);高等学校博士学科点专项科研基金项目(20110074130002) |
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| 摘 要： | 蒽是典型的多环芳烃类环境污染物,属于美国EPA优先控制的16种多环芳烃类化合物,其在高盐环境下的生物降解备受关注.本研究从某石油污染的高盐土壤中成功筛选出了1株高效降解蒽的菌株,经过对其生理生化特征和16S rDNA序列分析,初步鉴定并命名该菌株为Martelella sp.AD-3.该菌株在0.1%～10%的盐度和6.0～10.0的pH范围内,均能够降解蒽.其生长和降解蒽的优化条件是:蒽初始浓度25 mg·L-1、温度30℃、pH值9.0和盐度3%,在优化条件下培养6 d,蒽的降解率可达到94.6%.根据已报道的双加氧酶α亚基的同源性设计简并引物,通过巢式PCR扩增获得双加氧酶基因的部分序列307 bp(GenBank:JF823991.1),与海杆菌属Marinobacter sp.NCE312(AF295033)菌株萘双加氧酶大亚基的部分氨基酸序列同源性最高为95%.
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| 关 键 词： | 中度嗜盐菌 多环芳烃 蒽 生物降解 双加氧酶 |
| 收稿时间： | 2012/2/16 0:00:00 |
| 修稿时间： | 4/9/2012 12:00:00 AM |
Isolation, Charcaterization of an Anthracene Degrading Bacterium Martelella sp. AD-3 and Cloning of Dioxygenase Gene |
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| CUI Chang-zheng,FENG Tian-cai,YU Ya-qi,DONG Fei,YANG Xin-mei,FENG Yao-yu,LIU Yong-di and LIN Han-ping.Isolation, Charcaterization of an Anthracene Degrading Bacterium Martelella sp. AD-3 and Cloning of Dioxygenase Gene[J].Chinese Journal of Environmental Science,2012,33(11):4062-4068. |
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| Authors: | CUI Chang-zheng FENG Tian-cai YU Ya-qi DONG Fei YANG Xin-mei FENG Yao-yu LIU Yong-di and LIN Han-ping |
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| Institution: | State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;Patent Examination Cooperation Center of The Patent Office, State Intellectual Property Office of China, Beijing 100081, China;State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China;State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China |
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| Abstract: | |
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| Keywords: | moderately halophilic bacteria polycyclic aromatic hydrocarbons anthracene biodegradation dioxygenase |
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