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| 扑草净降解菌的分离、筛选与鉴定及降解特性初步研究 | |
| 引用本文： | 周际海,孙向武,胡锋,李辉信.扑草净降解菌的分离、筛选与鉴定及降解特性初步研究[J].环境科学,2013,34(7):2894-2898. |
| 作者姓名： | 周际海 孙向武 胡锋 李辉信 |
| 作者单位： | 1. 常州大学环境与安全工程学院,常州 213164;中国科学院土壤与农业可持续发展国家重点实验室,南京 210008;南京农业大学资源与环境科学学院,南京210095 2. 常州大学环境与安全工程学院,常州,213164 3. 南京农业大学资源与环境科学学院,南京,210095 |
| 基金项目： | 国家自然科学基金项目 (41271270);中国科学院土壤与农业可持续发展国家重点实验室开放基金项目 (0812201236);常州市基础研究计划项目 (20120026);常州大学科研启动基金项目 (ZMF1102070) |
| 摘 要： | 从安徽农药厂废水处理系统的活性污泥中筛选扑草净(prometryne)降解菌.通过富集驯化和选择性培养筛选纯化细菌,分离到2株细菌,分别命名为P-1、P-2.根据表型特征、生理生化特性和16S rDNA序列同源性分析:P-1为G-,鉴定为苍白杆菌属(Ochrobactrum);P-2为G+,鉴定为芽胞杆菌属(Bacillus).GenBank登录号分别为HM004554和HM004555.通过12 d液体降解实验,菌株P-1、P-2对40 mg.L-1扑草净降解率分别达到46.5%和65.4%.该研究为扑草净的生物降解与污染土壤的生物修复提供了依据. |
| 关 键 词： | 扑草净 微生物降解 鉴定 苍白杆菌属 芽胞杆菌属 |
| 收稿时间： | 2012/10/11 0:00:00 |
| 修稿时间： | 2012/12/26 0:00:00 |
Isolation, Screening and Identification of Prometryne-Degrading Bacteria and Their Degrading Characteristics |
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| ZHOU Ji-hai,SUN Xiang-wu,HU Feng and LI Hui-xin.Isolation, Screening and Identification of Prometryne-Degrading Bacteria and Their Degrading Characteristics[J].Chinese Journal of Environmental Science,2013,34(7):2894-2898. | |
| Authors: | ZHOU Ji-hai SUN Xiang-wu HU Feng and LI Hui-xin |
| Institution: | School of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, China;State Key Laboratory of Soil and Sustainable Agriculture, Chinese Academy of Sciences, Nanjing 210008, China;College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;School of Environmental & Safety Engineering, Changzhou University, Changzhou 213164, China;College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China |
| Abstract: | The purpose of this study was to develop some bacterial strains that could degrade prometryne effectively. Bacteria were screened and purified from sediments of a pesticide plant with the method of domestication with gradient concentrations. Two strains were isolated and named P-1 and P-2, respectively. According to their morphology, physiological, biochemical properties and the analysis of their 16S rRNA gene sequences, the strain P-1 is Gram negative and identified as Ochrobactrum; the strain P-2 is Gram positive and identified as Bacillus. GenBank Accession numbers are HM004554 and HM004555, respectively. Degradation rates of the strains P-1 and P-2 for prometryne concentration with 40 mg·L-1 were 46.5% and 65.4% after 12 days in the liquid culture experiment, respectively. This study provides an essential material and theoretical foundation for the remediation of prometryne contaminated soils. |
| Keywords: | prometryne biodegradation identification Ochrobactrum Bacillus |
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