| 四溴双酚A好氧降解菌的筛选及其降解特性研究 |
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| 引用本文： | 钱艳园,刘莉莉,于晓娟,丁丛,王志平,施玉衡,李晨君.四溴双酚A好氧降解菌的筛选及其降解特性研究[J].环境科学,2012,33(11):3962-3966. |
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| 作者姓名： | 钱艳园 刘莉莉 于晓娟 丁丛 王志平 施玉衡 李晨君 |
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| 作者单位： | 1. 上海交通大学环境科学与工程学院,上海,200240
2. 华东理工大学国家环境保护化工过程环境风险评价与控制重点实验室,上海,200237 |
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| 基金项目： | 国家自然科学基金项目(41001316,51108262) |
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| 摘 要： | 为了解四溴双酚A(TBBPA)的微生物降解特性,采用TBBPA为单一碳源的选择性培养基从苯酚降解颗粒污泥中筛选出4株对TBBPA具有良好降解性的好氧菌株,其中菌株H经16S rDNA测序鉴定为红球菌属.正交试验得出其最优降解条件为:温度30℃,摇床转速150 r.min-1,pH 6.5,腐殖酸浓度200 mg·L-1,K+浓度1 000 mg·L-1;此条件下21 d降解脱溴率达20.75%.LC-MS结果表明,TBBPA好氧降解的主要产物是分子中异丙基与苯环断裂后脱溴产生的一溴苯酚.聚丙烯酰胺蛋白凝胶电泳(SDS-PAGE)结果表明,该菌株H的蛋白系列中含有一条介于(90～117)×103间的条带,对比同期考察的葡萄糖培养与TBBPA无机盐溶液培养菌,结合粗酶液的降解实验,该蛋白可能是受TBBPA激活的特异性降解酶.
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| 关 键 词： | 四溴双酚A 红球菌 好氧降解 降解产物 降解酶 |
| 收稿时间： | 2012/1/13 0:00:00 |
| 修稿时间： | 2012/2/15 0:00:00 |
Isolation of Aerobic Degrading Strains for TBBPA and the Properties of Biodegradation |
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| QIAN Yan-yuan,LIU Li-li,YU Xiao-juan,DING Cong,WANG Zhi-ping,SHI Yu-heng and LI Chen-jun.Isolation of Aerobic Degrading Strains for TBBPA and the Properties of Biodegradation[J].Chinese Journal of Environmental Science,2012,33(11):3962-3966. |
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| Authors: | QIAN Yan-yuan LIU Li-li YU Xiao-juan DING Cong WANG Zhi-ping SHI Yu-heng and LI Chen-jun |
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| Institution: | School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China |
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| Abstract: | |
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| Keywords: | tetrabromobisphenol A Rhodococcus sp aerobic degradation degradation products degrading enzyme |
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