含硫芳香族化合物降解酶的定域及胞内产物的鉴定

为研究含硫芳香族化合物的生物降解途径 ,采用静息细胞技术提取假单胞杆菌 (Pseudomonas sp.)不同部位的酶 .结果表明 ,对硝基苯砜基乙酸异丁酯降解酶主要定域在细胞膜周及膜内 .被试化合物在膜内酶的作用下 ,发生碳硫键断裂 ,降解产物是对硝基苯磺酸 .在膜周酶的作用下 ,发生碳硫键和酯的烷氧键 2种方式的断裂 ,生成对硝基苯磺酸和对硝基苯砜基乙酸 .

Localization of Degradation Enzyme in Pseudomonas sp. for an Aromatic SulfurContaining Compound and Identification of Products in Cells

In order to study the biodegradation way of an aromatic sulfurcontaining compound,the resting cell technique was used to get different enzyme.The experimental results show that the degradation enzyme for 4-NO₂-Ph-SO₂-CH₂-CO-OC₄H₉-i mainly locates in cell and on the membrane.The enzyme in cells converts the tested compound to 4-NO₂Ph-SO₃H with bond cleavage between carbon and sulfur.By the enzyme located on the membrane the compound is degraded as 4-NO₂Ph-SO₃H and 4-NO₂Ph-SO₂-CH₂-COOH.The later is yielded with the bond cleavage between carbonyl and alkoxy group.