城市尾水氮代谢过程中芽孢杆菌对微藻作用机制

为了对城市尾水深度脱氮，提高地表水体质量，分别利用微藻（Z）、芽孢杆菌（Y）和微藻芽孢杆菌（ZY）对城市尾水进行脱氮处理与氮代谢菌群特征研究，结果表明，Z和ZY对城市尾水中氨氮的去除效果较好，2组降解率都达到了95%以上.Z对亚硝态氮去除效果最好，芽孢杆菌与微藻的共同作用在氮循环反应中亚硝态氮转化为硝态氮过程发挥出较为强大的稳定效果.芽孢杆菌可以有效去除硝态氮，并可以提高微藻对硝态氮的去除效率.ZY菌对城市尾水中硝态氮的去除效果最好，其降解率高达99%以上，几乎完全去除城市尾水中的硝态氮.在Z中样本数占比较高的菌群分别为Chroococcidiopsis_PCC_7203 （24.38%）、uncultured_bacterium-g_norank_f_A4b （23.65%）、Exiguobacteriu （7.09%）、Leptolyngbya_PCC-6306 （9.41%）和Bacillus （1.99%）.在ZY中样本数占比较高的菌群分别为Brevibacillus （22.94%）、Clostridium（8.78%）和Bacillus （4.88%），Chroococcidiopsis_PCC_7203 样本数占比仅为7.84%，远远低于Z样本数所占比例.微藻可以很好去除系统中氨氮，芽孢杆菌与微藻联用具有较好的氨氮和硝态氮去除效果，芽孢杆菌具有抑制微藻过度增长作用，防止水体富营养化和黑臭发生.研究可为城市尾水深度处理，防止地表水体富营养化提供数据支持.

Action Mechanism of Bacillus on Microalgae During Nitrogen Metabolism in Urban Tailwater

To improve deep denitrification of black and odorous water and improve the quality of surface water, we studied the characteristics of nitrogen metabolism and denitrification of urban tailwater by microalgae (Z), Bacillus (Y), and Bacillus microalgae (ZY). The results showed that there was a good removal effect of ammonia nitrogen of group Z and group ZY in urban tailwater. The degradation rate of both groups reached 95%. The best effect on the removal of nitrite nitrogen was of group Z in urban tailwater. The combined action of Bacillus and Micrococcus played a relatively strong and stable effect on the conversion of nitrite nitrogen to nitrate nitrogen in the nitrogen cycle reaction. Bacillus could effectively remove nitrate nitrogen and improve the removal efficiency of nitrate nitrogen by microalgae. Best removal effect of nitrate nitrogen was observed in group ZY in urban tailwater, with a degradation rate as high as 99%, in which the nitrate nitrogen was removed almost completely. The Bacteria with high proportions in Z were Chroococcidiopsis_PCC_7203 (24.38%), uncultured_bacterium-g_norank_f_A4b (23.65%), Exiguobacteriu (7.09%), Leptolyngbya_PCC-6306(9.41%), and Bacillus (1.99%). The bacteria with high proportions in ZY were Brevibacillus (22.94%), Clostridium (8.78%), and Bacillus (4.88%), and the proportion of Chroococcidiopsis_PCC_7203 was only 7.84% in ZY, which was considerably lower than that in Z samples. The conclusions were as follows:microalgae could effectively remove ammonia nitrogen in the system. Bacillus and microalgae had very good removal effect of ammonia nitrogen and nitrate nitrogen. During the nitrogen removal of black and odorous water by algae, the Bacillus inhibited the excessive growth of microalgae and prevented eutrophication and black odor in water. This study can provide data support for the deep treatment of urban tail water and prevention of surface water eutrophication.