鲁氏酵母菌WY-3降解甲胺磷的性能

从污水中分离出一株酵母菌,编号WY-3.该菌能利用甲胺磷(MAP)作唯一氮、磷源生长,也能利用甲胺、乙胺和硫酸铵(硝酸盐和羟胺除外)作氮源生长,它还能适应高浓度(60%)葡萄糖的培养条件,初步鉴定为鲁氏酵母菌(Saccharomyces rouxii).从WY-3菌无细胞抽提液中检测到一种高活力酸性磷酸酶,采用聚丙烯酰胺凝胶电泳活性染色法呈现一条白色谱带.氟化钠试剂可抑制该酶催化MAP释放无机磷,但是不影响该菌代谢MAP脱氨基的性能.MAP经WY-3菌作用后毒性会大大降低,分析其残留物时发现有无机磷和甲醇物质.此外,文中还对酵母菌一类共代谢菌降解MAP的途径进行了探讨.

Degradation of Methamidophos by Saccharomyces rouxii WY-3

A strain of Yeast WY-3 was isolated from wastewater sample.It is capable of utilizing methamidophos as sole nitrogen and phosphorus sources,and also capable of utilizing methylamine,ethylamine and ammonium sulfate as nitrogen sources except nitrate and hydroxylamine.The yeast could grow in medinm containing 60% glucose and was identified as Saccharomyces rouxii WY-3.The strain contains a high active acid phosphatase.The crude enzyme was applied to a plate of polyacrylanide gel for electrophoresis,then activity was detected as white single band.Inhibiting test showed that sodium fluoride could seriously inhibit the activity of acid phosphatase to release phosphorus from methamidophos,wherease it make no effect on deamination of the strain WY-3 from methamidophos.After methamidophos was degraded by strain WY-,toxity of the pesiticide reduced obviously,and its portion intermediate,methanol and inorganic phosphorus,were deteted.