蒽降解菌烟曲霉A10的分离及降解性能研究

从污染环境中筛选出1株蒽降解菌A10，经鉴定为烟曲霉（Aspergillus fumigatus），其对蒽的降解率随时间的延长逐渐升高，在12～84 h，蒽降解率增长速率较快；此后降解率的增加趋于平缓，最终（168 h）能够达到83%左右.当无机盐培养液中蒽初始浓度为10 mg/L，A10投菌量为50 g/L（以湿重计），菌龄为36 h时， 5 d内蒽降解率为79.37%.蒽浓度对菌发挥降解作用有较大影响，浓度为5 mg/L时，降解率最高，达92.17%.培养液初始pH为5.0～7.5时，降解率维持在60%左右；温度为30℃、氧气量为4.30 mg/L时蒽降解效果较好.一定量的营养盐的添加能在一定程度上促进蒽的降解.共代谢底物乳糖的添加，能使蒽的降解率提高37.15%.对蒽降解过程的初步研究表明，菌株A10对蒽的降解是一个胞外吸附/胞内降解的动态变化过程.红外光谱分析显示，在微生物作用下，蒽的结构发生改变，生成了含有1～2个苯环的芳香酸、芳香酮、芳香醛和饱和碳氢化合物等一系列降解产物.

Isolation of an Anthracene-Degrading Strain Aspergillus fumigatus A10 and Its Degradation Characteristics

An anthracene-degrading strain(A10) was isolated from contaminated environment and identified as Aspergillus fumigatus.The experimental results showed that the biodegradation rate of anthracene increased with the increasing time.Between 12-84 h interval,the biodegradation performed rapidly,while after this,the increase of biodegradation rate tended to become slow,and ultimately the biodegradation rate could achieve approximately 83%.The degradation rate of anthracene reached 79.37% within 5 days when the in...