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光合细菌与酵母跨界融合子降解味精废水性能测定
引用本文:程树培,崔益斌,夏伏虎,邓良伟,葛岚,王玉水.光合细菌与酵母跨界融合子降解味精废水性能测定[J].环境科学,1996,17(3):5-7.
作者姓名:程树培  崔益斌  夏伏虎  邓良伟  葛岚  王玉水
作者单位:南京大学环境科学与工程系
摘    要:为了筛选出能综合双亲优势的杂种细胞,为废资源化生产SCP提供更为理想的菌株来源,将原核球形红假单胞菌细胞与真核酿酒酵母细胞的原生质体融合,构建成跨界杂种细胞。从中筛选出F13,F15,F20三株高絮凝性融合细胞,在起始BOD5浓度为191-690mg/L,pH7.0,30℃的味精废水中振荡反应7-8h测得融合细胞双亲菌株降解利用有机污染物的系列的反应动力学参数μmax,qmax,Ksμ,Ksq,Y

关 键 词:酿酒酵母  原生质体融合  味精废水  光合细菌
收稿时间:1995/9/26 0:00:00

Function Determination for Biodegradation of Monosodium Glutamate(MSG)Wastewater with Fusants between Photosynthetic Bacteria and Yeast
Cheng Shupei.Function Determination for Biodegradation of Monosodium Glutamate(MSG)Wastewater with Fusants between Photosynthetic Bacteria and Yeast[J].Chinese Journal of Environmental Science,1996,17(3):5-7.
Authors:Cheng Shupei
Institution:Dept. of Environ. Sci. and Eng., Naming University, Naming 210093;Dept. of Environ. Sci. and Eng., Naming University, Naming 210093;Dept. of Environ. Sci. and Eng., Naming University, Naming 210093;Dept. of Environ. Sci. and Eng., Naming University, Naming 210093;Dept. of Environ. Sci. and Eng., Naming University, Naming 210093;Dept. of Environ. Sci. and Eng., Naming University, Naming 210093
Abstract:he protoplasts of Rhodopseudomonas sphaerndes(eukaryote,NtrSms)and Saccharomyces cerevisiae (prokaryote, NtsSmr)were induced with PEG(MW= 6000)to fuse for the construction of the fusant cell (NtrSmr).The fusants F13,F15 and F20 screened from the'fusion production with high flocculation were used to test the kinetics parameters for degradation of MSG wastewater in the shaking reaction for 7-8 h,while the initial BOD5 concentration of the wastewater were from 191 to 690 mg/L.The values of maximum specific growth rate umax. and its half velocity constant Ksu,the maximum specific degradation rate qmaxand its half velocity constant Ksq the true growth yield coefficient Y and the endogenous decay rate coefficient Kd were measured in the reaction.The results of this research suggest that the fusants F13,F15 and F20 have better ability for degradation.of the organic waste in MSG wastewater.
Keywords:Rh  sphaeroides  S  cerevisiae fusant  monosodium glutamate wastewater  biodegradation kinetic  
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