| 重金属污染农田生菜根际重金属固定细菌群落组成及其阻控效应 |
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| 引用本文： | 王铁军,苏楠楠,雷鹏,邱明洋,陈兆进,姚伦广,韩辉.重金属污染农田生菜根际重金属固定细菌群落组成及其阻控效应[J].环境科学,2019,40(11):5133-5141. |
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| 作者姓名： | 王铁军 苏楠楠 雷鹏 邱明洋 陈兆进 姚伦广 韩辉 |
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| 作者单位： | 南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061,南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061,南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061,南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061,南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061,南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061,南阳师范学院农业工程学院, 南水北调中线水源区水安全河南省协同创新中心, 南阳 473061 |
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| 基金项目： | 国家自然科学基金项目（41601332）；河南省科技攻关计划项目（172102110108） |
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| 摘 要： | 为了了解高含量重金属对蔬菜根际土壤可培养细菌和重金属固定细菌群落的影响,发掘其中的微生物资源,从河南省新乡市郊区采集一份高含量重金属污染(HY)和一份低含量重金属污染(DK)意大利生菜根际土壤,通过可培养分离技术和溶液吸附实验比较其重金属固定细菌的群落差异,通过水培实验研究功能菌株对生菜生长和富集Cd和Pb的影响.从HY和DK样品中各分离出400株细菌:HY样品中的400株细菌隶属于3门14属,其中β-Proteobacteria是优势门,而DK样品中的400株细菌隶属于4门30属,Firmicutes是优势门.HY样品中的400株细菌中有146株细菌对Cd和Pb的吸附率均大于80%,Brevundimonas、Serratia、Arthrobacter和Pseudarthrobacter为主要的属.而DK样品中的400株细菌中有44株细菌对Cd和Pb的吸附率均大于80%,Bacillus为主要的属.与不接菌对照相比,重金属固定细菌Serratia liquefaciens HY-22、Bacillus thuringensis HY-53和Acinetobacter lwoffii HY-157均能显著提高意大利生菜和花叶生菜根部(7. 5%～77. 6%)和地上部(15. 4%～67. 2%)干重,降低意大利生菜根部和地上部Cd(38. 7%～66. 6%)和Pb(34. 7%～62. 5%)的含量,此外Bacillus thuringensis HY-53能够使意大利生菜和花叶生菜新鲜叶片中Cd和Pb的含量达到食品安全国家标准.研究结果为Cd和Pb污染农田的修复和农作物的安全生产提供菌种资源和理论依据.
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| 关 键 词： | 重金属固定细菌 生菜 群落结构 钝化 吸附 |
| 收稿时间： | 2019/6/8 0:00:00 |
| 修稿时间： | 2019/6/18 0:00:00 |
Community Structure of Heavy Metal Immobilized Bacteria in the Lettuce (Lactuca sativa L.) Rhizosphere in Soil Polluted by Heavy Metals and Its Effects on Reducing Heavy Metal Accumulation in Lettuce |
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| WANG Tie-jun,SU Nan-nan,LEI Peng,QIU Ming-yang,CHEN Zhao-jin,YAO Lun-guang and HAN Hui.Community Structure of Heavy Metal Immobilized Bacteria in the Lettuce (Lactuca sativa L.) Rhizosphere in Soil Polluted by Heavy Metals and Its Effects on Reducing Heavy Metal Accumulation in Lettuce[J].Chinese Journal of Environmental Science,2019,40(11):5133-5141. |
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| Authors: | WANG Tie-jun SU Nan-nan LEI Peng QIU Ming-yang CHEN Zhao-jin YAO Lun-guang and HAN Hui |
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| Institution: | Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China,Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China and Collaborative Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, School of Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China |
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| Abstract: | |
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| Keywords: | heavy metal-immobilizing bacteria lettuce community structure immobilization absorb |
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