渤黄海夏季表层海水中好氧不产氧光合细菌多样性分析

于2011年8月采集渤黄海表层海水样品,利用编码光合反应复合中心小亚基的pufM基因构建克隆文库,比较分析了不同生境类型海区夏季表层海水中好氧不产氧光合细菌(aerobic anoxygenic phototrophic bacteria,AAPB)的多样性。结果发现,属于-3变形菌门(Proteobacteria)的红杆菌科(Rhodobacteraceae)在研究海区普遍存在,而且丰度较高,是研究海区AAPB的优势类群。其中渤海站位的AAPB 群落结构相对较丰富,含有-3、-4、-6 Proteobacteria 3个细菌类群;而渤海海峡站位和北黄海站位的AAPB群落结构相对单一,只有-3 Proteobacteria一个类群。另外,研究表明温度最高,盐度最低的渤海站位更有利于AAPB群落的生长及组成状态。测序结果显示所占比例最大的细菌类群为未知类群,表明本文所研究的海区中可能存在着大量未知的AAPB种类,因此有进一步研究开发的价值。

The diversity of aerobic anoxygenic phototrophic bacteria in surface seawaters in Bohai and Yellow Sea in summer

Biodiversity of the functional bacteria in surface water (0.5 m) in summer in Bohai Sea(BH), Bohai Strait (BHW) and North Yellow (BHH) Sea was studied by 16S rDNA clone library. Ecological distribution of aerobic anoxygenic phototrophic bacteria(AAPB) of the three typical sites in BH, BHW and BHH was analysed by clone libraries of RbcS-mc pufM gene which coding photosynthetic reaction composite center. The results showed that Rhodobacteraceae of -3 Proteobacteria with high relative abundance was the dominant AAPB bacterial flora which was prevalent in the study area. Among the three typical sites, the AAPB microbial community structure of site BH was relatively abundant, including -3、-4、-6 Proteobacteria; Sites BHW and BHH were relatively unitary, both contained -3 Proteobacteria. In addition, research has shown that the BH area was the highest summer temperature and minimum salinity in the three study sites, more conducive to the growth of AAPB community and state. Unidentified bacteria had the highest proportion which suggested that an abundance of unknown AAPB bacterial flora were existed in this sea area, there are worthy of further research and development.