黄渤海刺参稳定同位素组成特征的初步研究

本文应用元素分析仪及稳定同位素质谱仪联用技术对黄渤海刺参的碳、氮稳定同位素组成进行分析，同时，通过潜在食物源对刺参生长的贡献率进行计算，研究两海域刺参稳定同位素组成特征及其原因。刺参碳、氮稳定同位素测定结果显示，渤海刺参δ13C值、δ15N值的分布范围分别为-15.660‰~-12.027‰和7.463‰~11.238‰;黄海刺参δ13C值、δ15N值的分布范围分别为-16.062‰~-12.527‰和3.966‰~7.243‰。两海域刺参δ15N值分布范围存在明显差异，表明δ15N值能够有效区分黄渤海不同刺参样品。食源贡献率计算结果显示，渤海刺参食源相对贡献率依次为浮游生物、SOM、POM、海带和孔石莼;黄海刺参食源相对贡献率依次为孔石莼、海带、POM、SOM和浮游生物。食源相对贡献率的不同为两海域刺参δ15N值的差异提供了依据。相关结论能为稳定同位素技术应用于刺参的产地溯源研究提供理论依据。

Preliminary research on the stable isotope composition characteristics of Apostichopus japonicus in the Bohai and Yellow Seas

In this study,the stable carbon and nitrogen isotopic compositions of Apostichopus japonicus in the Bohai Sea and the Yellow Sea were performed by elemental analyzer coupled to a stable isotope ratio mass spectrometry (EA-SIRMS).Moreover,the contribution rates of potential food sources to the growth of A.japonicus were also calculated in order to study the stable isotope composition characteristics of A.japonicus in different seas and analyze the reasons.Results showed that δ13C and δ15N values of A.japonicus from the Bohai Sea ranged from -15.660‰ to -12.027‰ and 7.463‰ to 11.238‰,while that of A.japonicus from the Yellow Sea ranged from -16.062‰ to -12.527‰ and 3.966‰ to 7.243‰,which indicate that δ15N could clearly distinguish samples of A.japonicus between the Bohai Sea and the Yellow Sea.The contribution rates of potential food sources to the growth of A.japonicus from the Bohai Sea in descending were Plankton,SOM,POM,Laminaria japonica and Ulva pertusa,while that in the Yellow Sea were Ulva pertusa, Laminaria japonica,POM,SOM and Plankton.The difference of contribution rates provided an explanation for the difference of δ15N of A.japonicus between the two seas.The conclusion in this study will provide a theoretical basis for tracing the geographical origin of A.japonicus using stable isotope ratio mass spectrometry technology.