旋链角毛藻和中肋骨条藻间的他感作用

选择两种常见硅藻——中肋骨条藻(Skeletonema costatum)和旋链角毛藻(Chaetoceros curvisetus)为研究对象,采用滤液培养的方法考察二者之间的他感效应,结果表明:f/2全营养培养条件下,旋链角毛藻指数后期无藻细胞滤液对中肋骨条藻的生长有显著抑制作用,抑制率随旋链角毛藻滤液比例的增大而增大,他感效应随作用时间消退后,中肋骨条藻生长速率提高,且随着旋链角毛藻滤液比例的增大,中肋骨条藻的细胞粒径有减小的趋势;中肋骨条藻指数后期无藻细胞滤液对旋链角毛藻的生长速率和细胞粒径均无影响,表明中肋骨条藻对旋链角毛藻无他感作用。本实验结果可为下一步提取他感物质、研究他感作用机理提供实验基础。

Allelopathic effects between Chaetoceros curvisetus and Skeletonema costatum

The two common types of diatom,respectively Chaetoceros curvisetus and Skeletonema costatum,were chosen to observe and study the allelopathic effect between them by means of cell-free filtrate culture.The observation showed that under the condition of enriched,cell-free filtrate of the C.curvisetus culture inhibited the growth of S.costatum significantly in the later exponential growth phase.The inhibition rate was related to the proportion of C.curvisetus cell-free filtrate in the culture,in addition the diameter of S.costatum was affected as well.Allelochemical degraded as time went on,and then the relative growth rate of S.costatum was increased.There was no influence on the relative growth rate and diameter of S.costatum by enriched C.curvisetus cell-free filtrate in the end of exponential growth phase,which showed that there was not allelopathic effect of S.costatum on C.curvisetus.The results provided the experimental basis for extracting allelochemical and researching allelopathic mechanism later.