苯并[a]芘(BaP)暴露对三疣梭子蟹(Portunus trituberculatus)P4501A1、P-gp、HSP70基因表达的影响

以三疣梭子蟹(Portunus trituberculatus)为实验对象,通过水体暴露方式对其进行BaP持续染毒,随后进行消除试验,利用荧光定量qPCR技术研究三疣梭子蟹肝脏中细胞色素P4501A1(P4501A1)、P-糖蛋白(P-gp)、热休克蛋白70(HSP70)等相关基因表达量随BaP暴露时间和暴露剂量的变化特征。结果显示:BaP暴露对P4501A1、P-gp、HSP70基因的表达均起诱导作用,且对P4501A1的诱导最为显著(P0.05);消除期间P4501A1、P-gp、HSP70基因的表达量显著降低,且P4501A1表达量变化更为明显(P0.05)。实验结果表明BaP对三疣梭子蟹肝脏P4501A1基因诱导作用最为显著,可作为BaP暴露的敏感生物标志物,用以监测水环境中多环芳烃的污染。

Effect of Benzo[a]pyrene on the P4501A1,P-glycoprotein and HSP70 expression in Portunus trituberculatus

The gene expression patterns of P4501A1, P-gp and HSP70 of Portunus trituberculatus were both measured using realtime fluorescence quantitative PCR during crab exposed to environmentally relevant concentration of Benzoa]pyrene (0.05, 0.15, 0.45 g/L, respectively) and the release of the test.The results showed that P451A1, P-gp and HSP70 mRNA could be induced, besides, the content of P4501A1 mRNA induced is more significantly(P0.05).The content of three genes mRNA were decrease markedly after the release of the test and the P4501A mRNA has more obvious changes.In a word, The gene expression of P4501A1, P-gp and HSP70 were increase at the beginning of Benzoa]pyrene exposure, and then decreased after the release of the test.The above data suggest that Benzoa]pyrene could induced high concentration of P451A1 mRNA in the liver of crab to monitor indicates contamination of organic pollutants in the water of PAHs.