| 浮游微藻携带和传播WSSV的研究 |
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| 引用本文： | 张家松,董双林,田相利,董云伟.浮游微藻携带和传播WSSV的研究[J].海洋环境科学,2007,26(3):221-224. |
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| 作者姓名： | 张家松 董双林 田相利 董云伟 |
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| 作者单位： | 中国海洋大学,海水养殖教育部重点实验室,山东,青岛,266003 |
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| 基金项目： | 国家科技攻关计划
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国家高技术研究发展计划(863计划) |
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| 摘 要： | 以对虾养殖池塘中常见的湛江等鞭金藻(Isochrysis zhanjiangensis)、亚心形扁藻(Platymonas subcordiformis)、盐藻(D unaliella salina)和中肋骨条藻(Skeletonema costatum)四种浮游微藻为实验对象,研究其是否可以携带和传播严重危害对虾养殖业的对虾白斑病病毒(WSSV).研究结果表明,这四种微藻和WSSV病毒粗提液混合后,经PCR检测发现在混合后96 h内微藻都可以携带WSSV,其中湛江等鞭金藻和亚心形扁藻在144 h内为WSSV阳性,盐藻在120 h内为阳性,然后在不同时间呈现WSSV阴性.采取病毒-浮游微藻吸附法,用携带WSSV的微藻投喂美丽猛水蚤(Nitocra sp.5d,经套式PCR检测,美丽猛水蚤为病毒阳性.这说明浮游微藻可以携带并且传播WSSV.
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| 关 键 词： | 浮游微藻 WSSV 携带 传播 浮游微藻 传播 WSSV 研究 spread four 套式 猛水蚤 投喂 吸附法 阴性 时间 阳性 发现 检测 混合 粗提液 结果 病毒 对虾白斑病 |
| 文章编号： | 1007-6336（2007）03-0221-04 |
| 收稿时间： | 2006-12-31 |
| 修稿时间： | 2006-12-312007-03-10 |
Study on four phytoplanktons carried and spread WSSV |
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| ZHANG Jia-song,DONG Shuang-lin,TIAN Xiang-li,DONG Yun-wei.Study on four phytoplanktons carried and spread WSSV[J].Marine Environmental Science,2007,26(3):221-224. |
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| Authors: | ZHANG Jia-song DONG Shuang-lin TIAN Xiang-li DONG Yun-wei |
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| Institution: | Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, China |
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| Abstract: | Phytoplankton is the base of the food web in pond cultures.In addition,they remove small particles in culture water,including viruses which are excreted by the infected animals,maintain the stable environment condition for culture.In present study,the possibility was studied that four microalgea(Isochrysis zhanjiangensis,Platymonas subcordiformis,D unaliella salina and Skeletonema costatum) may act as a carrier or vector of white spot syndrome virus.After mixed with the WSSV in column,it was found that the micro-algae could carry WSSV within 96 h.The harpacticoid copepod Nitocra sp.could infect WSSV by filtering the phytoplanktons which were carrying WSSV.The results showed that the phytoplankton could be a carrier and vector of white spot syndrome virus. |
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| Keywords: | phytoplankton WSSV carrier vector |
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