温度对中国鲎幼鲎生长、蜕壳、能值、免疫指标和抗氧化能力的影响研究

本文研究了温度（29℃、31℃、33℃、35℃和37℃）对中国鲎（Tachypleus tridentatus）幼鲎蜕壳同步性、机体免疫力和抗氧化能力等的影响。研究结果显示：（1）当温度高于33℃时幼鲎的成活率显著降低（P<0.05），蜕壳增重率随温度的升高而显著降低（P<0.05），蜕壳率则随着温度升高呈先显著升高（P<0.05）后显著降低（P<0.05）的趋势，33℃试验组蜕壳率最高；（2）温度较高试验组幼鲎开始蜕壳的时间较早，33℃试验组的幼鲎最先蜕壳；一、二龄幼鲎能值随温度升高而显著降低（P<0.05）;(3)37℃高温组一、二幼鲎的ACP活力均显著降低（P<0.05），而AKP活力变化没有明显规律；（4）一龄幼鲎CAT活性随温度升高而显著降低（P<0.05）,37℃试验组二龄幼鲎的TAOC和CAT活性均显著低于其他试验组（P<0.05）。研究结果表明，当温度低于33℃时，适当升高温度有利于中国鲎幼鲎的蜕壳与生长；当温度超过33℃时，幼鲎的免疫力、抗氧化能力显著降低，成活率下降。本文的结果可以为中国鲎人工育苗和放流活动开展提供基础...

Effects of temperature on growth,molting, emergy,immune index and antioxidation of juvenile Chinese horseshoe crab, Tachypleus tridentatus

The effects of different temperatures (29 ℃, 31 ℃, 33 ℃, 35 ℃ and 37 ℃) on the synchronization of molting, body immunity and oxidation resistance of juvenile Chinese horseshoe crab (Tachypleus tridentatus) were explored. The results showed that: (1) The survival rate decreased significantly when the temperature was higher than 33 ℃ (P<0.05). With the increase of temperature, the molting weight gain rate decreased significantly (P<0.05). The molting rate increased significantly at first and then decreased significantly with the increase of temperature (P<0.05) , and the 33 ℃ group was the highest. (2) The molting time of higher temperature test groups were earlier, and the 33 ℃ group was the first. The emergy of the two-stage juvenile horseshoe crabs tended to decrease with the increasing temperature. (3) The ACP activity of the horseshoe crabs at 37 ℃ was significantly lower than that of other groups (P<0.05), while the AKP activity had no obvious regularity.(4)With the increasing temperature, the CAT activities of the first-year-old horseshoe crabs significantly decreased. And the T-AOC and CAT activities of the 37 ℃ test group were significantly lower than those of other test groups (P<0.05). The results showed that when the temperature was lower than 33 ℃, raising the temperature properly was conducive to promote the molting and growth of juvenile horseshoe crabs. After exceeding 33 ℃, the immunity and oxidation resistance of juvenile horseshoe crabs decreased significantly, and the survival rate decreased. This study provides a theoretical basis for the artificial breeding and releasing of Chinese horseshoe crab.