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Effects of exogenous Ca2+ on the membrane permeability, MDA and SH group content of Alexandrium sp. LC3 under surfactant stress |
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| Authors: | LI Hao MIAO Jin-lai CUI Feng-xi LIU Xiao-guang and LI Guang-you |
| Institution: | 1. Marine Life Sciences College, Ocean University of China, Qingdao 266003,China;Key Laboratory of Marine Biological Active Substances, SOA, Qingdao 266061,China 2. Key Laboratory of Marine Biological Active Substances, SOA, Qingdao 266061,China 3. Fishery College,Ocean University of China,Qingdao 266003,China |
| Abstract: | The effect of Ca 2 on the removal of Alexandrium sp. LC3 under HDTMAB stress was investigated. The results showed that the toxic effect of HDTMAB on Alexandrium sp. LC3 was significantly reduced in the presence of Ca 2 , especially under 4 mmol/L of Ca 2 . To understand the underlying mechanism, the SH group and MDA content of the cell membrane and membrane permeability were measured. It was found that the SH content of cell member increased, the MDA content and membrane permeability decreased when Alexandrium sp. was treated with Ca 2 and HDTMAB complex, compared with using HDTMAB only. The data suggested that Ca 2 might promote HDTMAB stress resistance of Alexandrium sp. LC3 by reducing the permeability and increasing the stability of cell membrane. |
| Keywords: | Ca 2 surfactant Alexandrium sp LC3 cell membrane permeability |
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