| Oxidative stress and apoptosis of Carassius auratus lymphocytes induced by nonplanar （PCB153） and coplanar （PCB169） polychlorinated biphenyl congeners in vitro |
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| 作者姓名： | ZHANG Jianying ZHANG Hangjun NI Wanmin |
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| 作者单位： | ZHANG Jian-ying,NI Wan-min(College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China);ZHANG Hang-jun(College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China;College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China) |
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| 基金项目： | This work was supported by the Zhejiang Provincial
Natural Science Foundation of China (No. Z506039) and
the National Key Basic Research and Development Program
in China (No. 2002CB410810). |
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| 摘 要： | Among all the 209 kinds of polychlorinated biphenyls (PCBs) congeners, nonplanar and coplanar PCB congeners have di erent levels
of toxicity on mammal cells such as neuronal cells, but little is known about their toxicity on fish cells although PCB congeners usually
have high bioaccumulation abilities in the detected fish bodies. This study showed that 2,2’,4,4’,5,5’-hexacholorbiphenyl (PCB153,
nonplanar congener) and 3,3’,4,4’,5,5’-hexacholorbiphenyl (PCB169, coplanar congener) caused apoptosis on the isolated crucian carp
(Carassius auratus) lymphocytes and the induced cytotoxicity was structure-dependent. According to the laser confocal microscope
observations, apoptosis was clearly distinguished by condensation of nucleus, shrinkage and formation of apoptotic bodies. DNA
fragmentation was detected by agarose gel electrophoresis. These typical morphological and biochemical characteristics indicate the
occurrence of apoptosis on fish lymphocytes. According to the flow cytometry analysis, after the cells were exposed to 10 mol/L
PCBs for 3 h, the apoptotic percentage induced by PCB153 was 23.41%, while that induced by PCB169 was even higher (31.03%).
Furthermore, incubating PCBs with fish lymphocytes enhanced levels of reactive oxygen species (ROS) and malondialdehyde (MDA),
clearly indicating the presence of oxidative stress and lipid peroxidation. Our data also demonstrate that the di erent cytotoxic e ects
induced by coplanar and nonplanar PCBs were correlated with their structural characteristics and the coplanar congener was more
cytotoxic than nonplanar congener. This study suggests that cytotoxicity mechanisms of the PCB congeners on fish lymphocytes
depend on their planarity and chemical structures.
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| 关 键 词： | 细胞凋亡 多氯联苯 淋巴细胞 氧化应激 非平面 诱导 体外 共面 |
| 收稿时间： | 12 November 2008 |
| 修稿时间： | 4/9/2009 12:00:00 AM |
Oxidative stress and apoptosis of Carassius auratus lymphocytes induced
by nonplanar (PCB153) and coplanar (PCB169) polychlorinated
biphenyl congeners in vitro |
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| ZHANG Jianying,ZHANG Hangjun,NI Wanmin.Oxidative stress and apoptosis of Carassius auratus lymphocytes induced
by nonplanar (PCB153) and coplanar (PCB169) polychlorinated
biphenyl congeners in vitro[J].Journal of Environmental Sciences,2009,21(9):1284-1289. |
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| Authors: | ZHANG Jianying ZHANG Hangjun and NI Wanmin |
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| Institution: | 1. College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China
2. College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310028, China;College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China |
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| Abstract: | and the coplanar congener was more cytotoxic than nonplanar congener. This study suggests that cytotoxicity mechanisms of the PCB congeners on fish lymphocytes depend on their planarity and chemical structures. |
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| Keywords: | apoptosis lymphocytes malondialdehyde (MDA) reactive oxygen species (ROS) |
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