Using the nematode Caenorhabditis elegans as a model animal for assessing the
toxicity induced by microcystin-LR |
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Authors: | LI Yunhui WANG Yang YIN Lihong PU Yuepu and WANG Dayong |
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Institution: | 1. School of Public Health, Southeast University, Nanjing 210009, China;Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Nanjing 210009, China;Department of Genetics and Developmental Biology, Southeast University, Nanjing 210009, China 2. Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Nanjing 210009, China;School of Public Health, Southeast University, Nanjing 210009, China 3. Department of Genetics and Developmental Biology, Southeast University, Nanjing 210009, China |
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Abstract: | Among more than 75 variants of microcystin (MC), microcystin-LR (MC-LR) is one of the most common toxins. In this study, the feasibility of using Caenorhabditis elegans to evaluate MC-LR toxicity was studied. C. elegans was treated with MC-LR at different concentrations ranging from 0.1 to 80 μg/L. The results showed that MC-LR could reduce lifespan, delay development, lengthen generation times, decrease brood sizes, suppress locomotion behaviors, and decreases hsp-16-2-gfp expression. The endpoints of generation time, brood size, and percentage of the population expressing hsp16-2-gfp were very sensitive to 1.0 μg/L of MC-LR, and would be more useful for the evaluation of MC-LR toxicity. Furthermore, the tissue-specific hsp16-2-gfp expressions were investigated in MC-LR-exposed animals, and the nervous system and intestine were primarily effected by MC-LR. Therefore, the generation time, brood size, and hsp16-2-gfp expression in C. elegans can be explored to serve as valuable endpoints for evaluating the potential toxicity from MC-LR exposure. |
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Keywords: | microcystin-LR toxicity assessment brood size stress response Caenorhabditis elegans |
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