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Green synthesis, characterization and antimicrobial activity of silver nanoparticles using methanolic root extracts of Diospyros sylvatica
Authors:Lakshmi Pethakamsetty  Kalyani Kothapent  Hanumanta Rao Nammi  Lakshmi Kalyani Ruddaraju  Pratap Kollu  Soon Gil Yoon  Sri Venkata Narayana Pammi
Institution:1 Department of Microbiology, College of Science and Technology, Andhra University, Visakhapatnam 530003, India;2 DST-PURSE Programme Advanced Analytical Laboratory, Andhra University, Visakhapatnam 530003, India;3 Sri Vishnu College of Pharmacy, Department of Pharmaceutical Technology, Vishnupur, Bhimavaram, A.P. 530041, India;4 DST-INSPIRE Faculty, Department of Metallurgical Engineering & Materials Science, Indian Institute of Technology Bombay, Mumbai 400076, India;5 Department of Materials Science and Engineering, Chungnam National University, Daeduk Science Town, 305-764 Daejeon, South Korea
Abstract:The current research study focuses to formulate the biosynthesized silver nanoparticles for the first time from silver acetate using methanolic root extracts of Diospyros sylvatica, a member of family Ebenaceae. TEM analysis revealed the average diameter of Ag NPs around 8 nm which is in good agreement with the average crystallite size (10 nm) calculated from X-ray Diffraction (XRD) analysis. Further the study has been extended to the antimicrobial activity against test pathogenic Gram (+) ve, Gram (−) ve bacterial and fungal strains. The bioinspired Ag-NP showed promising activity against all the tested bacterial strains and the activity was enhanced with increased dosage levels.
Keywords:Root extract  Silver nanoparticles  Antimicrobial properties
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