| Determnation of ochratoxin A in grain by monoclonal antibody-based enzyme-linked immunosorbent assay |
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| 作者姓名： | Yang Chuanhe Luo Xueyun Liu Chang Li Wenyan Li Yiepeng Zhao Danyu Ji Rong |
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| 作者单位： | Institute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China,Institute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China,Institute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China,Institute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China,Institute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China,Institute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China,Institute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China |
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| 摘 要： | ＤｅｔｅｒｍｎａｔｉｏｎｏｆｏｃｈｒａｔｏｘｉｎＡｉｎｇｒａｉｎｂｙｍｏｎｏｃｌｏｎａｌａｎｔｉｂｏｄｙ－ｂａｓｅｄｅｎｚｙｍｅ－ｌｉｎｋｅｄｉｍｍｕｎｏｓｏｒｂｅｎｔａｓｓａｙＹａｎｇＣｈｕａｎｈｅ；ＬｕｏＸｕｅｙｕｎ；ＬｉｕＣｈａｎｇ；ＬｉＷｅｎ...
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| 关 键 词： | enzyme-linked immunosorbent assay (ELISA) ochratoxin A monoclonal antibody cereal. |
| 收稿时间： | 1992/4/13 0:00:00 |
Determnation of ochratoxin A in grain by monoclonal antibody-based enzyme-linked immunosorbent assay |
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| Yang Chuanhe,Luo Xueyun,Liu Chang,Li Wenyan,Li Yiepeng,Zhao Danyu,Ji Rong.Determnation of ochratoxin A in grain by monoclonal antibody-based enzyme-linked immunosorbent assay[J].Journal of Environmental Sciences,1994,6(1):116-122. |
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| Authors: | Yang Chuanhe Luo Xueyun Liu Chang Li Wenyan Li Yiepeng Zhao Danyu Ji RongInstitute of Food Safety Control and inspection Ministry of Public HealthBeijing China |
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| Institution: | Yang Chuanhe; Luo Xueyun; Liu Chang; Li Wenyan;Li Yiepeng; Zhao Danyu; Ji RongInstitute of Food Safety Control and inspection. Ministry of Public HealthBeijing 100021 . China |
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| Abstract: | The simple rapid and sensitive enzyme-linked immunosorbent assay (ELISA) methods, di-rect and indirect ELISA, for quantitation of ochratoxin A in cereal had been developed by theutilization of monoclonal antibody on immunomicroplate. Direct FLIAS was found to be less timeconsuming than indirect ELISA. For direct FLISA, recovery of 1 -500 ppb OA added to wheat was78.9-100.0% and rice was 88.9- 120.0%. For indirect EI.IAS, recovery of 1-500 ppb OA addedto wheat was 79.0- 110.0% and rice was 82.0 120.0%. The minimal detection level for OA was Ippb. Analyses of 31 samples that caused humanintoxicant for OA showed that the ELISA resultsagreed wtll with those obtained by thin-layer chromatogrdphy. |
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| Keywords: | enzyme-linked immunosorbent assay (ELISA) ochratoxin A monoclonal antibody cereal |
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