Evolution of urban black and odorous water: The characteristics of microbial community and driving-factors

Urban black blooms that are primarily caused by organic carbon are deleterious environ-mental problems.However,detailed studies on the microbial characteristics that form ur-ban black blooms are lacking.In this study,we observed the composition,diversity,and function of bacterial community in the overlying water and sediments during the occur-rence and remediation of urban black blooms using high-throughput 16S rRNA gene am-plicon sequencing analysis.First,we found that pivotal consortia in the overlying water increased significantly during the formation of black blooms,including the genera Acidovo-rax,Brevundimonas,Pusillimonas,and Burkholderiales involved in the degradation of refractory organics,as well as the genera Desulfovibrio,Dechloromonas,and Rhizobium related to the pro-duction of black and odorous substances.An RDA analysis revealed that chemical oxygen demand,dissolved oxygen,and oxidation reduction potential were related to the changes in microbial community composition.Furthermore,aeration was found to accelerate the removal of ammonia nitrogen and enhance the function of microbial community by stim-ulating the growth of order Planktomycetes during the remediation of black blooms,but aeration substantially damaged the microbial diversity and richness.Therefore,the health of the aquatic ecosystem should be comprehensively considered when aeration is applied to restore polluted waterbodies.Notably,we observed a large number of pathogenic bacteria in urban black blooms,which emphasizes the importance of treating domestic sewage so that it is harmless.Together,these findings provide new insights and a basis to prevent and manage urban black blooms.