| Determination of alkali-labile phosphoprotein phosphorus from fish plasma using the Tb^3＋-tiron complex as a fluorescence probe |
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| 作者姓名： | LV Xue-fei ZHAO Yi-bing ZHOU Qun-fang JIANG Gui-bin SONG Mao-yong |
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| 作者单位： | [1]State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beifing 100085, China [2]Department of Chemistry, the Key Laboratory of Analytical Sciences of Ministry of Education, Xiamen University, Xiamen 361005, China |
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| 基金项目： | 国家自然科学基金
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国家重点基础研究发展计划(973计划) |
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| 摘 要： | A sensitive method based on the fluorescence quenching effect of the Tb^3＋-Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus （ALP） released from fish plasma. The detection limit was 5.4 ng/ml （S/N=2）, and the relative standard deviation of the quenching effect （6 replicates） was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity.
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| 关 键 词： | 鱼浆 不耐碱磷蛋白 磷 测定 铽^3＋ 钛试剂 荧光探针 指示生物 |
| 收稿时间： | 2006-05-22 |
| 修稿时间： | 2006-08-08 |
Determination of alkali-labile phosphoprotein phosphorus from fish plasma
using the Tb3+-tiron complex as a fluorescence probe |
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| LV Xue-fei,ZHAO Yi-bing,ZHOU Qun-fang,JIANG Gui-bin,SONG Mao-yong.Determination of alkali-labile phosphoprotein phosphorus from fish plasma
using the Tb3+-tiron complex as a fluorescence probe[J].Journal of Environmental Sciences,2007,19(5):616-621. |
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| Authors: | LV Xue-fei ZHAO Yi-bing ZHOU Qun-fang JIANG Gui-bin and SONG Mao-yong |
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| Institution: | 1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
2. Department of Chemistry, the Key Laboratory of Analytical Sciences of Ministry of Education, Xiamen University, Xiamen 361005, China |
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| Abstract: | A sensitive method based on the fluorescence quenching effect of the Tb3 -Tiron complex is proposed for the determination of alkali-labile phosphoprotein phosphorus (ALP) released from fish plasma. The detection limit was 5.4 ng/ml (S/N=2), and the relative standard deviation of the quenching effect (6 replicates) was 4.6%. The results obtained by the proposed method were in good agreement with those obtained by the colorimetric assay. The advantages of the present method are its relatively simple detection procedure, the lack of toxic organic solvents, and high sensitivity. |
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| Keywords: | alkali-labile phosphoprotein phosphorus (ALP) Chinese loach fluorescence quenching Tb3 -Tiron complex vitellogenin plasma fish phosphorus Determination fluorescence probe complex high sensitivity lack organic solvents present simple procedure results good colorimetric assay relative standard deviation fluorescence quenching detection Tiron |
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