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Laboratory culture of the freshwater benthic gastropod Bellamya aeruginosa (Reeve) and its utility as a test species for sediment toxicity
作者姓名:Taowu M  Shuangjiao Gong  Ke Zhou  Cheng Zhu  Kaidong Deng  Qinghua Luo  Zijian Wang
作者单位:Taowu Ma,Shuangjiao Gong,Ke Zhou,Cheng Zhu,Kaidong Deng,Qinghua Luo(College of Biology and Environmental Science, Jishou University, Jishou 416000, China);Zijian Wang(State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China) 
基金项目:supported by the National Natural Science Foundation of China (No.20677021);;the Science Foundation of Jishou University (No.jsdxkyzz200101)
摘    要:This study aimed to develop original laboratory culture and sediment toxicity testing protocols for the freshwater gastropod Bellamya aeruginosa(Reeve),a new potential species for sediment toxicity testing.B.aeruginosa was successfully cultured with an effective culture system under proposed laboratory conditions.Optimal ad libitum feeding levels for larvae,juveniles,and adults were 2.0,6.0,and 16.0 mg fish food/(snail·day),respectively.Mean survival rates of juveniles were higher than 90%.The snails could be sexed at 9 weeks of age,and their generation time is approximately 4 months.Reproduction continued all year around;the mean fecundity was 0.55 newborn/(female·day).The utility of this species for bioassays was evaluated in both 10-day and 28-day case studies with artificial sediments.The 10-day LC50 of Cu for larvae was 480 μg/g dry weight(dw),and the lowest observed effects concentration of Cu for survival and growth of larvae was 195 μg/g dw.Survival and growth are reliable indicators of acute toxicity.Larvae accumulated more Cu than adults.B.aeruginosa exhibited a higher sensitivity to Cu exposure than standard test species(Hyalella azteca and Chironomus tentans).The 28-day test of sediment toxicity with adults showed that fecundity was a robust endpoint indicator of reproductive toxicity,and the biochemical endpoints of superoxide dismutase,catalase,and glutathione could be used as sensitive biomarkers for Cu-induced oxidative damage.B.aeruginosa can be therefore recommended as a candidate for the standardization of the freshwater sediment toxicity test protocol.

关 键 词:铜锈环棱螺  实验室培养  水沉积物  毒性测试  物种  超氧化物歧化酶  未成年人  幼虫生长
收稿时间:28 March 2009
修稿时间:9/3/2009 12:00:00 AM

Laboratory culture of the freshwater benthic gastropod Bellamya aeruginosa (Reeve) and its utility as a test species for sediment toxicity
Taowu M,Shuangjiao Gong,Ke Zhou,Cheng Zhu,Kaidong Deng,Qinghua Luo,Zijian Wang.Laboratory culture of the freshwater benthic gastropod Bellamya aeruginosa (Reeve) and its utility as a test species for sediment toxicity[J].Journal of Environmental Sciences,2010,22(2):304-313.
Authors:Taowu M  Shuangjiao Gong  Ke Zhou  Cheng Zhu  Kaidong Deng  Qinghua Luo and Zijian Wang
Institution:1. College of Biology and Environmental Science, Jishou University, Jishou 416000, China
2. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
Abstract:This study aimed to develop original laboratory culture and sediment toxicity testing protocols for the freshwater gastropod Bellamya aeruginosa (Reeve), a new potential species for sediment toxicity testing. B. aeruginosa was successfully cultured with an e ective culture system under proposed laboratory conditions. Optimal ad libitum feeding levels for larvae, juveniles, and adults were 2.0, 6.0, and 16.0 mg fish food/(snail day), respectively. Mean survival rates of juveniles were higher than 90%. The snails could be sexed at 9 weeks of age, and their generation time is approximately 4 months. Reproduction continued all year around; the mean fecundity was 0.55 newborn/(female day). The utility of this species for bioassays was evaluated in both 10-day and 28-day case studies with artificial sediments. The 10-day LC50 of Cu for larvae was 480 g/g dry weight (dw), and the lowest observed e ects concentration of Cu for survival and growth of larvae was 195 g/g dw. Survival and growth are reliable indicators of acute toxicity. Larvae accumulated more Cu than adults. B. aeruginosa exhibited a higher sensitivity to Cu exposure than standard test species (Hyalella azteca and Chironomus tentans). The 28-day test of sediment toxicity with adults showed that fecundity was a robust endpoint indicator of reproductive toxicity, and the biochemical endpoints of superoxide dismutase, catalase, and glutathione could be used as sensitive biomarkers for Cu-induced oxidative damage. B. aeruginosa can be therefore recommended as a candidate for the standardization of the freshwater sediment toxicity test protocol.
Keywords:gastropod  cultures  life history  lowest observed effect concentration  bioaccumulation  oxidative stress  
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