Diversity of methanotrophs in Zoige wetland soils under both anaerobic and
aerobic conditions |
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Authors: | Juanli Yun Anzhou M Yaoming Li Guoqiang Zhuang Yanfen Wang and Hongxun Zhang |
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Institution: | 1. Graduate University of Chinese Academy of Sciences,Beijing 100049,China 2. Research Center for Eco-Environment Sciences,Chinese Academy of Sciences,Beijing 100085,China |
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Abstract: | Zoige wetland is one of the most important methane emission centers in China. The oxidation of methane in the wetland a ects
global warming, soil ecology and atmospheric chemistry. Despite their global significance, microorganisms that consume methane in
Zoige wetland remain poorly characterized. In this study, we investigated methanotrophs diversity in soil samples from both anaerobic
site and aerobic site in Zoige wetland using pmoA gene as a molecular marker. The cloning library was constructed according to
the pmoA sequences detected. Four clusters of methanotrophs were detected. The phylogenetic tree showed that all four clusters
detected were a liated to type I methanotrophs. Two novel clusters (cluster 1, cluster 2) were found to relate to none of the recognized
genera of methanotrophs. These clusters have no cultured representatives and reveal an ecological adaptation of particular uncultured
methanotrophs in Zoige wetland. Two clusters were belonging to Methylobacter and Methylococcus separately. Denaturing gradient
gel electrophoresis gel bands pattern retrieved from these two samples revealed that the community compositions of anaerobic soil and
aerobic soil were di erent from each other while anaerobic soil showed a higher metanotrophs diversity. Real-time PCR assays of the
two samples demonstrated that aerobic soil sample in Zoige wetland was 1.5 times as much copy numbers as anaerobic soil. These data
illustrated that methanotrophs are a group of microorganisms influence the methane consumption in Zoige wetland. |
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Keywords: | cloning library denaturing gradient gel electrophoresis methanotrophs pmoA gene real-time PCR Zoige wetland |
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