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Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere
Authors:Likun Gu  Zhihui Bai  Bo Jin  Qing Hu  Huili Wang  Guoqiang Zhuang and Hongxun Zhang
Institution:1. Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China;Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beij
2. Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
3. Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;School of Earth and Environment Sciences, The University of Adelaide, Adelaide, SA 5005, Australia
4. Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China;Graduate University of Chinese Academy of Sciences, Beijing 100039, China
Abstract:Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were ...
Keywords:fungicide  phyllosphere  microbial community  16S rDNA clone library  PCR-DGGE  
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