Profiling of the assembly of RecA nucleofilaments implies a potential target for environmental factors to disturb DNA repair |
| |
Authors: | Zheng Yuan Fangzhi Yu Dapeng Zhang Hailin Wang |
| |
Institution: | 1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;2. University of Chinese Academy of Sciences, Beijing 100049, China;3. Institute of Environment and Health, Jianghan University, Wuhan 430056, China;4. Institute of Environment and Health, Hangzhou Institute for Advanced Study, UCAS, Hangzhou 430056, China |
| |
Abstract: | Double-strand breaks (DSBs), one class of the most harmful DNA damage forms that bring elevated health risks, need to be repaired timely and effectively. However, an increasing number of environmental pollutants have been identified to impair DSB repair from various mechanisms. Our previous work indicated that the formation of unsaturated RecA nucleofilaments plays an essential role in homology recombination (HR) pathway which can accurately repair DSBs. In this study, by developing a benzonase cutting protection assay and combining it with traditional electrophoretic mobility shift assay (EMSA) analysis, we further investigated the assembly patterns of four RecA mutants that display differential DSB repair ability and ATPase activity. We observed that the mutants (G204S and S69G) possessing both ATP hydrolysis and DSB repair activities form unsaturated nucleofilaments similar to that formed by the wild type RecA, whereas the other two ATP hydrolysis-deficient mutants (K72R and E96D) that fail to mediate HR form more compacted nucleofilaments in the presence of ATP. These results establish a coupling of ATPase activity and effective DSB repair ability via the assembly status of RecA nucleofilaments. This linkage provides a potential target for environmental factors to disturb the essential HR pathway for DSB repair by suppressing the ATPase activity and altering the assembly pattern of nucleofilaments. |
| |
Keywords: | Corresponding author DNA double-strand breaks (DSBs) RecA assembly Homology recombination (HR) ATP hydrolysis DNA damage repair Environmental health |
|
| 点击此处可从《环境科学学报(英文版)》浏览原始摘要信息 |
| 点击此处可从《环境科学学报(英文版)》下载免费的PDF全文 |
|