Using fluorescence surrogates to track algogenic dissolved organic matter (AOM) during growth and coagulation/flocculation processes of green algae

Tracking the variation of the algogenic organic matter(AOM) released during the proliferation of green algae and subsequent treatment processes is crucial for constructing and optimizing control strategies. In this study, the potential of the spectroscopic tool was fully explored as a surrogate of AOM upon the cultivation of green algae and subsequent coagulation/flocculation(C/F) treatment processes using ZrCl_4 and Al_2(SO_4)_3. Fluorescence excitation emission matrix coupled with parallel factor analysis(EEM-PARAFAC) identified the presence of three independent fluorescent components in AOM, including protein-like(C1), fulvic-like(C2) and humic-like components(C3). Size exclusion chromatography(SEC) revealed that C1 in AOM was composed of large-sized proteins and aromatic amino acids. The individual components exhibited their unique characteristics with respect to the dynamic changes. C1 showed the highest correlation with AOM concentrations(R~2= 0.843) upon the C/F processes. C1 could also be suggested as an optical predictor for the formation of trihalomethanes upon the C/F processes. This study sheds a light for the potential application of the protein-like component(C1) as a practical surrogate to track the evolution of AOM in water treatment or wastewater reclamation systems involving Chlorella vulgaris green algae.