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Stable-isotope probing of bacterial community for dissolved inorganic carbon utilization in Microcystis aeruginosa-dominated eutrophic water
Authors:Weiguo Zhang  Jiangye Li  Chengcheng Wang  Xue Zhou  Yan Gao  Zhongjun Jia
Institution:1. Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2. Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Nanjing 210014, China;3. China Ministry of Agriculture Key Laboratory at Yangtze River Plain for Agricultural Environment, Nanjing 210014, China;4. College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China;5. College of Water Conservancy and Hydropower Engineering, Hohai University, Nanjing 210098, China;6. Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
Abstract:Dissolved inorganic carbon(DIC) is an important source of carbon in aquatic ecosystems,especially under conditions of increased frequency of cyanobacterial bloom. However, the importance of bacteria in direct or indirect utilization of DIC has been widely overlooked in eutrophic freshwater. To identify the functional bacteria that can actively utilize DIC in eutrophic freshwater during cyanobacterial bloom, stable-isotope probing(SIP) experiments were conducted on eutrophic river water with or without inoculation with cyanobacteria(Microcystis aeruginosa). Our 16 S rRNA sequencing results revealed the significance of Betaproteobacteria, with similar relative abundance as Alphaproteobacteria, in the active assimilation of H~(13)CO~(3-) into their DNA directly or indirectly, which include autotrophic genera Betaproteobacterial ammonia-oxidizing bacteria. Other bacterial groups containing autotrophic members, e.g. Planctomycetes and Nitrospira, also presented higher abundance among free-living bacteria in water without cyanobacteria. Microcystis aggregates showed a preference for some specific bacterial members that may utilize H~(13)CO~(3-) metabolized by Microcystis as organic matter, e.g. Bacteroidetes(Cytophagales, Sphingobacteriales), and microcystindegrading bacteria Betaproteobacteria(Paucibacter/Burkholderiaceae). This study provides some valuable information regarding the functional bacteria that can actively utilize DIC in eutrophic freshwater.
Keywords:Dissolved inorganic carbon  Stable isotope probing  Eutrophication  Corresponding author at: Institute of Agricultural Resources and Environment  Jiangsu Academy of Agricultural Sciences  Nanjing 210014  China  
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