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Construction of hybrid cell with Phanerochaete chrysosporium todegrade terephthalic acid wastewater (I) phenotype evidence
作者姓名:CHENG Shu-pei  ZHONG Zhi-hui  LU Peng  CHEN Jun  ZHU Jian-zhong  JUEZi-long
作者单位:1. The National Key Laboratory of Pollution Control and Resource Reuse,Department of Environmental Sciences and Engineering,Na
基金项目:TheNationalNaturalScienceFoundationofChina !(No .395 70 10 1)andNSFofJiangsuProvince (No .BK 990 33)
摘    要:IntroductionPhanerochaetechrysosporium (PC)isoneoftheunusualwhiterotfungithatitisabletomineralizethecompoundofnativelignin .Anditalsocandegradealmostallhazardousorganicpollutantsincludinggeneticmutagenicagentssuchaschlorinatedorganiccompounds,polycyclic…

收稿时间:1999/8/12 0:00:00
修稿时间:1999/10/20 0:00:00

Construction of hybrid cell with Phanerochaete chrysosporium todegrade terephthalic acid wastewater (I) phenotype evidence
CHENG Shu-pei,ZHONG Zhi-hui,LU Peng,CHEN Jun,ZHU Jian-zhong,JUEZi-long.Construction of hybrid cell with Phanerochaete chrysosporium todegrade terephthalic acid wastewater (I) phenotype evidence[J].Journal of Environmental Sciences,2000,12(4):394-397.
Authors:CHENG Shu-pei  ZHONG Zhi-hui  LU Peng  CHEN Jun  ZHU Jian-zhong  JUEZi-long
Abstract:The fungi Phanerochaete chrysosporium (PC) and Saccharomyces cerevisiae Y99 and the native bacterium YZ1 were the three parental strains for construction of hybrid cells through protoplast fusion to degrade terephthalic acid (TPA) wastewater. The results showed that the native bacterium YZ1 protoplasm could integrate with that of PC to form the hybrid cell Fhh and the fungus Y99 protoplasm also could integrate with that of Fhh to form the hybrid cell Fhhh. The protoplasts of YZ1 and Y99 could change the morphology of PC spore and mycelium for two times. The hybrid cell Fhhh got the best growth and degradation abilities in the wastewater. It suggested that the hybrid strains obtained from the inter\|kingdom protoplast fusion of the three parental strains could create potential for the purification of TPA wastewater.
Keywords:Phanerochaete chrysosporium  YZ1 bacterium  Saccharomyces cerevisiae  protoplast fusion  terephthalic acid wastewater
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