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Anaerobic biodegradation of benzene series compounds by mixed cultures based on optional electronic acceptors
Authors:HU Zhi-feng  DOU Jun-feng  LIU Xiang  ZHENG Xi-lai and DENG Dong
Institution:1. College of Environmental Science and Engineering,Ocean University of China,Qingdao 266003,China
2. Department of Environmental Science and Engineering,Tsinghua University,Beijing 100084,China
Abstract:A series of batch experiments were performed using mixed bacterial consortia to investigate biodegradation performance of benzene,toluene,ethylbenzene and three xylene isomers (BTEX) under nitrate,sulfate and ferric iron reducing conditions.The results showed that toluene,ethylbenzeoe,m-xylene and o-xylene could be degraded independently by the mixed cultures coupled to nitrate,sulfate and ferric iron reduction.Under ferric iron reducing conditions the biodegradation of benzene and p-xylene could be occurred only in the presence of other alkylbenzenes.Alkylbenzenes can serve as the primary substrates to stimulate the transformation of benzene and p-xylene under anaerobic conditions.Benzene and p-xylene are more toxic than toluene and ethylbenzene,under the three terminal electron acceptors conditions,the degradation rates decreased with toluene > ethylbenzene > m-xylene > o-xylene > benzene > p-xylene.Nitrate was a more favorable electron acceptor compared to sulfate and ferric iron.The ratio between sulfate consumed and the loss of benzene,toluene,ethylbenzene,o-xylene,m-xylene,p-xylene was 4.44,4.51,4.42,4.32,4.37 and 4.23,respectively;the ratio between nitrate consumed and the loss of these substrates was 7.53,6.24,6.49,7.28,7.81,7.61,respectively;the ratio between the consumption of ferric iron and the loss of toluene,ethylbenzene,o-xylene,m-xylenewas 17.99,18.04,18.07,17.97,respectively.
Keywords:benzene  toluene  ethylbenzene and three xylene isomers(BTEX)  anaerobic biodegradation  nitrate reduction  sulfate reduction  ferric iron reduction  electronic  based  mixed  compounds  series  benzene  biodegradation  consumption  ratio  loss  electron acceptor  substrates  terminal  serve  primary  stimulate  transformation  anaerobic  presence  reduction
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