首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Phylogenetically clustering of rhizobia by genome structure: application to unclassified Rhizobium
Authors:ZHENG Jun-fang  LIU Gui-rong and LIU Shu-lin
Institution:1. Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;Department of Microbiology, Peking University Health Science Center, Beijing 100083, China
2. Department of Microbiology, Peking University Health Science Center, Beijing 100083, China
Abstract:Previous research reveals that the genome structures of rhizobial type strains and reference strains can reflect their phylogenetic relationships. In order to further explore the potential application of genome structure as a phylogenetic marker in rhizobial natural taxonomy, this study analyzed the genome structures of 29 unclassified nodule bacteria isolated from the root nodules of leguminous trees, Robinia sp., Dalbergia spp., and A lbizia spp. and 7 rhizobial reference strains by I-CeuI cleavage, then clustered these bacteria phylogenetically based on their genome structures and compared these clusters with those based on numerical taxonomy and 16S rDNA PCR-RFLP. Eleven phylogenetic clusters were obtained, The clusters were in large part consistent with those based on numerical taxonomy and 16S rDNA PCR-RFLP. Also there are inconsistent clusters based on the above three methods. But results are completely consistent with 16S rRNA clusters. This suggested that the genome structure clustering method can be used to lastly identify root nodule isolates and detect their phylogenetic relationships. The credibility and repeatability of the results, together with the simplicity and possibility to analyze a large number of strains in a short time of the method, indicates the broad potential application of genome structure as phylogenetic marker to categorize rhizobial isolates and should in the future facilitate biodiversity studies.
Keywords:rhizobia  genome structure  phylogenetic analysis  pulsed field gel electrophoresis  I-CeuI
本文献已被 CNKI 维普 万方数据 PubMed 等数据库收录!
点击此处可从《环境科学学报(英文版)》浏览原始摘要信息
点击此处可从《环境科学学报(英文版)》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号