Aerobic degradation of methyl tert-butyl ether by a Proteobacteria strain in a closed culture system |
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Authors: | ZHONG Wei-hong CHEN Jian-meng LU Zheng CHEN Dong-zhi and CHEN Xiao |
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Institution: | College of Biological and Environmental Engineering,Zhejiang University of Technology,Hangzhou 310032,China |
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Abstract: | The contamination of methyl tert-butyl ether (MTBE) in underground waters has become a widely concerned problem all over the world. In this study, a novel closed culture system with oxygen supplied by H2O2 was introduced for MTBE aerobic biodegradation. After 7 d, almost all MTBE was degraded by a pure culture, a member of β-Proteobacteria named as PM1, in a closed system with oxygen supply, while only 40% MTBE was degraded in one without oxygen supply. Dissolved oxygen (DO) levels of the broth in closed systems respectively with and without H2O2 were about 5-6 and 4 mg/L. Higher DO may improve the activity of monooxygemase, which is the key enzyme of metabolic pathway from MTBE to tert-butyl alcohol and finally to CO2, and may result in the increase of the degrading activity of PM1 cell. The purge and trap GC-MS result of the broth in closed systems showed that tert-butyl alcohol,isopronol and acetone were the main intermediate products. |
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Keywords: | methyl tert-butyl ether biodegradation β-Proteobacteria intermediate products systems culture closed system strain ether methyl degradation acetone intermediate products purge and trap result increase cell key enzyme pathway alcohol improve activity levels |
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