Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances

Aluminum flocculant can enhance the flocculating performance of activated sludge.However,the binding mechanism of aluminum ion(Al 3+) and extracellular polymeric substances(EPS) in activated sludge is unclear due to the complexity of EPS.In this work,threedimensional excitation emission matrix fluorescence spectroscopy(3DEEM),fluorescence quenching titration and Fourier transform infrared spectroscopy(FT-IR) were used to explore the binding behavior and mechanism between Al 3+ and EPS.The results showed that two fluorescence peaks of tyrosineand tryptophan-like substances were identified in the loosely bound-extracellular polymeric substances(LB-EPS),and three peaks of tyrosine-,tryptophanand humic-like substances were identified in the tightly boundextracellular polymeric substances(TB-EPS).It was found that these fluorescence peaks could be quenched with Al 3+ at the dosage of 3.0 mg/L,which demonstrated that strong interactions took place between the EPS and Al 3+.The conditional stability constants for Al 3+ and EPS were determined by the Stern-Volmer equation.As to the binding mechanism,the-OH,N-H,C=O,C-N groups and the sulfurand phosphorus-containing groups showed complexation action,although the groups in the LB-EPS and TB-EPS showed different behavior.The TB-EPS have stronger binding ability to Al 3+ than the LB-EPS,and TB-EPS play an important role in the interaction with Al 3+.

Flocculating characteristic of activated sludge flocs: Interaction between Al3+ and extracellular polymeric substances

Aluminum flocculant can enhance the flocculating performance of activated sludge. However, the binding mechanism of aluminum ion (Al³⁺) and extracellular polymeric substances (EPS) in activated sludge is unclear due to the complexity of EPS. In this work, three-dimensional excitation emission matrix fluorescence spectroscopy (3DEEM), fluorescence quenching titration and Fourier transform infrared spectroscopy (FT-IR) were used to explore the binding behavior and mechanism between Al³⁺ and EPS. The results showed that two fluorescence peaks of tyrosine- and tryptophan-like substances were identified in the loosely bound-extracellular polymeric substances (LB-EPS), and three peaks of tyrosine-, tryptophan- and humic-like substances were identified in the tightly bound-extracellular polymeric substances (TB-EPS). It was found that these fluorescence peaks could be quenched with Al³⁺ at the dosage of 3.0 mg/L, which demonstrated that strong interactions took place between the EPS and Al³⁺. The conditional stability constants for Al³⁺ and EPS were determined by the Stern-Volmer equation. As to the binding mechanism, the -OH, N-H, C=O, C-N groups and the sulfur- and phosphorus-containing groups showed complexation action, although the groups in the LB-EPS and TB-EPS showed different behavior. The TB-EPS have stronger binding ability to Al³⁺ than the LB-EPS, and TB-EPS play an important role in the interaction with Al³⁺.