Construction of WCB-11: A novel phiYFP arsenic-resistant
whole-cell biosensor |
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Authors: | Qing Hu Li Li Yanjuan Wang Wenjuan Zhao Hongyan Qi and Guoqiang Zhuang |
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Institution: | Department of Environmental Bio-Technology,Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China |
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Abstract: | The prediction and assessment of environmental pollution by arsenic are important preconditions of advocating environmental
protection and human health risk assessment. A yellow fluorescent protein-based whole-cell biosensor for the detection of arsenite and
arsenate was constructed and tested. An arsenic-resistant promoter and the regulatory gene arsR were obtained by PCR from the genome
of Escherichia coli DH5 , and phiYFP was introduced into E. coli DH5 as a reporter gene to construct an arsenic-resistant whole-cell
biosensor (WCB-11) in which phiYFP was expressed well for the first time. Experimental results demonstrated that the biosensor has a
good response to arsenic and the expression of phiYFP. When strain WCB-11 was exposed to As3+ and As5+, the expression of yellow
fluorescence was time-dependent and dose-dependent. This engineered construct is expected to become established as an inexpensive
and convenient method for the detection of arsenic in the field. |
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Keywords: | arsenic reporter gene whole-cell biosensor yellow fluorescent protein (phiYFP) |
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