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Real-time fluorescent quantitative immuno-PCR method for determination of fluoranthene in water samples with a molecular beacon |
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| Authors: | Qiyan Ye Huisheng Zhuang Chun Zhou Qionge Wang |
| Institution: | 1. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China 2. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China;School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China 3. College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanhai 201620, China |
| Abstract: | A reliable and sensitive competitive real-time fluorescent quantitative immuno-PCR (RTFQ-IPCR) assay using a molecular beacon was developed for the determination of trace fluoranthene (FL) in the environment. Under optimized assay conditions, FL can be determined in the concentration range from 1 fg/mL to 100 ng/mL, with y = 0.194x + 7.859, and a correlation coefficient of 0.967 was identified, with a detection limit of 0.6 fg/mL. Environmental water samples were successfully analyzed, recovery was between 90% and 116%, with intra-day relative standard deviation (RSD) of 6.7%-12.8% and inter-day RSD of 8.4%-15.2%. The results obtained from RTFQ-IPCR were confirmed by ELISA, showing good accuracy and suitability to analyze FL in field samples. As a highly sensitive method, the molecular beacon-based RTFQ-IPCR is acceptable and promising for providing reliable test results to make environmental decisions. |
| Keywords: | fluoranthene real-time fluorescent quantitative immuno-PCR molecular beacon |
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